Research integrity

Pier Paolo and Pier Giuseppe, the titans of IFOM-IEO

Meet two grand cancer researchers from Milan: Pier Paolo Di Fiore and Pier Giuseppe Pelicci. Then decide if you want to give them your tax and charity money.

This post is dedicated to the IFOM-IEO-Campus, a cancer research centre in Milan, Italy, where I used to work as postdoc for over 4.5 years. The campus is small in area, but tightly packed with research labs and people from all over the world, heavily funded by international grants and Italian charity money. It is run by two institutions, IEO (Istituto Europea di Oncologia) and IFOM ( Istituto FIRC di Oncologia Molecolare, FIRC being a cancer research foundation, my affiliation back then was with IFOM). Aside of that separation, everything is fluid there: facilities are shared and people constantly move employment between IFOM and IEO, as in fact even the former IFOM director, Pier-Paolo Di Fiore, did. He is the main character of this article, together with another IEO bigwig, Pier Guiseppe Pelicci. The two men are incredibly influential in Italian science, and publish in the most prestigious research journals. What to make of their research achievements, is another question.

I know many people mentioned below personally, and in fact I am very disappointed at what I discovered on PubPeer. One case I even covered before, in this article. During my time in Milan, I met many great scientists at IFOM-IEO Campus, yet not many of them progressed anywhere beyond eternal postdoc, while their less qualified, less diligent, or even less honest colleagues became group leaders and tenured principal investigators. Also Di Fiore and Pelicci themselves trained new generations of group leaders, and recruited other group leaders to IFOM-IEO based on certain criteria, these people in turn are now training the PIs of the future. It seems, the only thing which counted were papers in Nature, Cell and Science, less so how the results were generated, or if they were reproducible even in your own lab.

Research integrity and diligence seem to be an obscure concept at IFOM-IEO, in fact one wonders if some of these sceintists grasp it at all. My own then-boss, Fabrizio d’Adda di Fagagna, apparently remains staunchly supportive of his long-term collaborator, the misconduct-tainted German zombie scientist Karl Lenhard Rudolph, despite everything. The d’Adda di Fagagna lab recently published a paper Michelini et al Nature Cell Biology 2017 where a dataset was possibly improperly reused:

It is a money scheme, where hot claims are made in order to attract even more charity funding, much of it via direct advertising to convince people to send 5 per mille of their salaries to IEO or IFOM. This is actually why Di Fiore, Pelicci and others were recently reported to Milanese state prosecutor, more on that at the end. In any case, the money scheme needs papers in high impact factor journals, and that sometimes means heavy bullshitting. The cases I present below contain evidence of more than just bullshitting, namely of actual data manipulation, or research fraud if you like. It is up to IFOM-IEO leaders to find the culprits, or scapegoats. Much of the evidence was posted on PubPeer by the pseudonymous Clare Francis, some of it just recently. I present here a selection, please visit PubPeer for more discussion of Di Fiore’s and Pelicci’s papers.

Let us start with a 17 year old paper in Nature which surely made Di Fiore big, back then.

Simona Polo, Sara Sigismund, Mario Faretta, Monica Guidi, Maria Rosaria Capua, Giovanna Bossi, Hong Chen, Pietro De Camilli, Pier Paolo Di Fiore

A single motif responsible for ubiquitin recognition and monoubiquitination in endocytic proteins

Nature (2002) doi: 10.1038/416451a 

This Nature paper also helped Simona Polo establish herself as group leader in di Fiore’s IFOM. Also the second author Sara Sigismund is presently group leader under Di Fiore at IEO. Another mentee of Di Fiore is IFOM group leader Giorgio Scita, together with his boss, Scita published this in 1999:

Giorgio Scita, Johan Nordstrom, Roberta Carbone, Pierluigi Tenca, Giuseppina Giardina, Silvio Gutkind , Mattias Bjarnegård, Christer Betsholtz, Pier Paolo Di Fiore

EPS8 and E3B1 transduce signals from Ras to Rac

Nature (1999) doi: 10.1038/45822

Followed by this 2004 paper as last author:

Nina Offenhäuser, Alessandro Borgonovo, Andrea Disanza, Pascale Romano, Isabella Ponzanelli, Gioacchin Iannolo, Pier Paolo Di Fiore, Giorgio Scita

The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway

Molecular Biology of the Cell (2004) doi: 10.1091/mbc.e03-06-0427 


Nina Offenhäuser is now senior manager at Cogentech, a biotech spin-off located inside the IFOM-IEO Campus, among which directors is the current IFOM director, Marco Foiani.

This western blot in yet another Di Fiore Nature paper also looks suspicious:

Letizia Lanzetti, Andrea Palamidessi, Liliana Areces, Giorgio Scita, Pier Paolo Di Fiore

Rab5 is a signalling GTPase involved in actin remodelling by receptor tyrosine kinases

Nature (2004) doi: 10.1038/nature02542 


Scita is also coauthor there, but he did not yet comment on PubPeer about that 2004 Nature paper. He did comment on another, even older paper, and shared what we are told is the original raw data:

Giorgio Scita, Pierluigi Tenca, Liliana B. Areces, Arianna Tocchetti, Emanuela Frittoli, Giuseppina Giardina, Isabella Ponzanelli, Patrizia Sini, Metello Innocenti, Pier Paolo Di Fiore

An effector region in Eps8 is responsible for the activation of the Rac-specific GEF activity of Sos-1 and for the proper localization of the Rac-based actin–polymerizing machine

Journal of Cell Biology (2001) doi: 10.1083/jcb.200103146 

Figure 7E, flagged for possible gel band duplication. On the right, a comparison of figure panels with the raw data provided by Scita.

The raw data Scita uploaded on 1 August 2019 is of very low resolution, which might serve as a red flag. Another scientist, Gary Stacey, submitted low-resolution “raw data” images to counter accusations of band duplication. Obviously it makes no sense why original image files only exist in very poor quality, while higher-resolution images of same gels were submitted to journal for publication. But in any case, Scita’s raw data meant to prove that the allegedly duplicated bands are different. The two Sos-1 and HA bands above sure look different in the low-res data files, but: they do not match the corresponding bands published in JCB 2001. Their neighbour bands match nicely, so it does seem Scita found the original image film. How could this have happened? Surely not because someone played with the raw data after being alerted to a suspected band duplication, hence also the low resolution of the provided scans? There must be a different explanation, maybe Scita can provide it.

This raw data dissonance seems to happen also in another case in the same paper, also there Scita submitted ultra-low resolution “raw data”:

Evidence from PubPeer alleging a band duplication, with “raw data” provided by Scita as comparison. Does it really match the published figure?

Make up your own mind what this means for research integrity at IFOM. As aside, the Milanese star of cancer research Pier Paolo Di Fiore is originally from Naples, which might explain his past collaboration with the local fraud kingpin there, the unsackable Alfredo Fusco. Together, Fusco and Di Fiore published in the 1980ies and 1990ies 15 papers, two of which have been retracted for gross data manipulation.

But now back to the IFOM-IEO-Campus in Milan. The following paper from there features Di Fiore as coauthor, and Pelicci, the grand man of IEO, as last author.

Myriam Alcalay, Natalia Meani, Vania Gelmetti, Anna Fantozzi , Marta Fagioli , Annette Orleth , Daniela Riganelli, Carla Sebastiani, Enrico Cappelli, Cristina Casciari , Maria Teresa Sciurpi, Angela Rosa Mariano, Simone Paolo Minardi, Lucilla Luzi, Heiko Muller , Pier Paolo Di Fiore, Guido Frosina, Pier Giuseppe Pelicci

Acute myeloid leukemia fusion proteins deregulate genes involved in stem cell maintenance and DNA repair

Journal of Clinical Investigation (2003) doi: 10.1172/jci17595 


Small fragments in the last two gel lanes seem to bee duplicated, as if some unwanted signal had to be retouched.

Myriam Alcalay, presently director of Experimental Haematology Unit at IEO, pretended on PubPeer not to understand the concerns:

“it is difficult to infer what they are pointing to with the boxes and arrows. […] The faint bands indicated by Petrocephalus and Pseudanapaea are clearly non-specific bands, also present in other lanes, that in no way interfere with the specific bands or alter the significance of the experiment. The result was also confirmed with a different technique (comet assay) as shown in figure 4c. “

The following was probably one of Pelicci’s big papers which helped establish his massive power at IEO and its mothership, the Fondazione Umberto Veronesi.

Luciano Di Croce, Veronica A Raker , Massimo Corsaro, Francesco Fazi, Mirco Fanelli , Mario Faretta, Francois Fuks, Francesco Lo Coco, Tony Kouzarides, Clara Nervi, Saverio Minucci , Pier Giuseppe Pelicci

Methyltransferase recruitment and DNA hypermethylation of target promoters by an oncogenic transcription factor

Science (2002) doi: 10.1126/science.1065173

This is really to plump. No wonder Science prefers to leave that old paper alone.

The penultimate author Saverio Minucci is now director of Chromatin Alteration Unit at IEO, while first author Luciano Di Croce moved on from IEO to become group leader at CRG in Barcelona, a place famous for protecting an Italian Photoshop artist, Maria Pia Cosma.

Pelicci’s family also works at IEO: both his wife, Luisa Lanfrancone and his sister, Guiliana Pelicci, are PIs there. This was published by the brother and sister team:

Giuliana Pelicci , Flavia Troglio, Alessandra Bodini, Rosa Marina Melillo, Valentina Pettirossi, Laura Coda, Antonio De Giuseppe, Massimo Santoro , Pier Giuseppe Pelicci

The neuron-specific Rai (ShcC) adaptor protein inhibits apoptosis by coupling Ret to the phosphatidylinositol 3-kinase/Akt signaling pathway

Molecular and Cellular Biology (2002) doi: 10.1128/mcb.22.20.7351-7363.2002 

PubPeer explanation: ” Figure 1D. Please compare lanes 1 and 2 of the right WB:alpha-Shc panel with a horizontal mirror image of the left WB: alpha-Shc panel. In addition to the duplication/mirror image there is also a duplication of the “empty” lanes in 1D WB:alpha-Grb2 (see the pair of dots above the latter “W”, and the same pair of dots above the letter “b”). “

Those were some of Pelicci’s own papers, and it seems diligent research takes a back seat in his lab when the goal is to publish high. Here Pelicci’s coauthor and IEO researcher Enrica Migliaccio posted on PubPeer evidence of a loading control library practice, i.e. when the loading control is run on a separate gel (a discussion about western blot loading controls here and here). The paper was Gambino et al Aging Cell 2013, it was then corrected.

Pelicci’s collaborative works are also impressive. This was published by Pelicci and Minucci in collaboration with the lab of Nicoletta Landsberger, from the Ospedale San Raffaele in Milan. The first author Simona Segalla is presently postdoc at the same Milanese research hospital.

Simona Segalla , Laura Rinaldi, Charlotte Kilstrup-Nielsen , Gianfranco Badaracco, Saverio Minucci, Pier Giuseppe Pelicci , Nicoletta Landsberger

Retinoic acid receptor alpha fusion to PML affects its transcriptional and chromatin-remodeling properties

Molecular and Cellular Biology (2003) doi: 10.1128/mcb.23.23.8795-8808.2003 

There is much more of cloned gel bands and gel lanes in that train-wreck of a paper, for example this:

The following paper was made at Pelicci’s IEO, the last author being the Belgian researcher Jean-Christophe Marine, then at IEO. The paper shows the PhD research of the first author, Davide Danovi, done at IEO.

Davide Danovi , Erik Meulmeester , Diego Pasini, Domenico Migliorini, Maria Capra, Ruth Frenk , Petra De Graaf , Sarah Francoz, Patrizia Gasparini, Alberto Gobbi , Kristian Helin, Pier Giuseppe Pelicci, Aart G Jochemsen , Jean-Christophe Marine

Amplification of Mdmx (or Mdm4) directly contributes to tumor formation by inhibiting p53 tumor suppressor activity

Molecular and Cellular Biology (2004) doi: 10.1128/mcb.24.13.5835-5843.2004 

The paper was criticised for undisclosed splicing, where the loading control library gel image was used to support the downregulation of two proteins. Marine explained that ” when this work was published (more than 15 years ago), this journal did not request to explicitly indicate whether the blots were composite neither to provide the uncropped blots”, and ended the debate with:

“The only remaining questions and/or remarks can no longer be addressed and/or are irrelevant. No additional comments needed. Thanks to all for your consideration and time. “

So a reader of my site showed me this image duplication (on the left), and then posted it on PubPeer. One cannot really reuse an image by mistake while rotating, cropping, darkening and resizing it.

Marine and his IEO coauthors had to correct the paper, and they even had to admit gel splicing in Figure 5 which Marine declared irrelevant and none of anyone’s business. The correction also states:

“These results were generated well over 15 years ago from samples at the European Institute of Oncology tissue microarray facility, which has subsequently been restructured and relocated. After numerous attempts, we have failed to identify how these mistakes were created or locate the original underlying data. This does not undermine the work or the conclusions of our study, which have been extensively cited and validated.”

There is of course more to show from IFOM-IEO-Campus, but this article is getting too long. Maybe I should briefly mention Francesco Blasi (who aged 82 still hasn’t thought of retiring), with 17 papers on PubPeer. He published things like this in Mazzieri et al Mol Biol Cell 2006:

Or this, in Bernardi et al PLOS One 2010. What kind of attitude does this suggest, from the people in the lab, and from the PI who seems not to care?

A Franken-Gel from Blasi lab at IFOM

Maybe also the former IEO researcher Kristian Helin should be mentioned here, with 13 papers on Pubpeer. Helin moved back to Denmark 15 years ago to become director of BRIC, the Copenhagen cancer research institute which now hosts another Photoshop artist, Janine Erler. As director, Helin was personally accused of silencing whistleblowers in the Erler scandal, and even being involved in the massive-scale destruction of the whistleblowers’ research material. Some months ago, BRIC decided to appoint a new director, and Helin announced to concentrate on his new job in New York, though he retains his professorship in Copenhagen.

The following example is from Helin’s paper Bracken et al EMBO J 2003, done back at IEO. The first lanes for SUZ12 are most likely duplicated, not that anyone bothered to investigate so far.

Things are starting to move however. As the newspaper Il Fatto Quotidiano reported, the Italian state prosecutor now investigates allegations of charity money embezzlement in connection with research fraud in Milan (free article version here):

“The suspects professors are Pier Paolo Di Fiore (from IFOM […]), Alberto Mantovani (from Humanitas Institute for research and care by the Rocca family), Pier Giuseppe Pelicci (from IEO, the European Institute of Oncology, founded by Umberto Veronesi), Marco Pierotti, Maria Angela Greco, Elena Tamburini and Silvana Pilotti (from the Istituto nazionale dei tumori). They maneuvered millions of funding from the Ministry of Research, the Ministry of Health, from Istituto superiore di sanità, from the CNR (Consiglio nazionale delle ricerche). They received just for the period analysed by the prosecution, between 2005 and 2012, such high figures: €9.37 mn Di Fiore; €3.06 mn Mantovani; €1.48 mn Pelicci; €3.6 mn Pierotti. “From the first analysis of Milan research centers by the judicial police,” writes the prosecutor, “nine freely available publications emerged which contain manipulations, more or less serious and showing images of the alleged experiments.” Other investigations discovered “additional 17 publications containing image manipulations”. Then the two scientific advisors appointed by the Milan prosecutor’s office analysed “159 scientific articles related to authors in discussion and containing images obtained by the technique of gel electrophoresis.” Once you have narrowed the field to a smaller sample, they concluded that “the 32 of the 25 scientific publications analysed were the subject to manipulation.”

Alberto Mantovani, who is EMBO member and professor at the private medical school Humanitas University in Milan, has at least 9 papers listed on PubPeer (not to be confused with another Milanese cancer researcher, Roberto Mantovani, who has his own PubPeer record). Mantovani sent in 2016 this statement explaining the concerns in 6 of his papers to his employer Humanitas University, the university was satisfied and “no problem was raised on their side”. The Humanitas professor also contacted the journals. Here is his letter to PNAS:

Marco Pierotti is not just somebody, but actually director of the Istituto Nazionale Tumori, and as such a major heavyweight in Italian cancer research. 26 of his papers are listed on PubPeer, and this PubPeer evidence apparently formed the basis of the report submitted to Milanese state prosecutor, as well as for Mantovani, Pelicci and Di Fiore. Let’s wait how it plays out. This public declaration was issued by Di Fiore and Pelicci in July 2019, quote:

“We feel obliged and in need to reject firmly the allegations of scientific fraud allegations and to assert towards the scientific community and the public our total scientific and moral integrity.
The charges brought against us refer to allegedly fraudulent manipulations of scientific images (mainly alleged duplication of images during the assembly of the later published figures).
We have produced a large dossier of documentary material, including the available original data from which the disputed images were derived, with which we are ready to reaffirm the total absence of a behaviour in any way related to scientific fraud.”

This article has been updated


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27 comments on “Pier Paolo and Pier Giuseppe, the titans of IFOM-IEO

  1. The cartoon (and artwork in general) is wonderful. I love the way you make him appear a bit fatter than he really is, cackling away in pleasure.

    Like

    • Yes, I think he lost weight some years ago. Which is good, I guess. Less good is PPDF’s propensity to make female subordinates cry, but apparently this was a virile leadership trait much cherished at IFOM-IEO. Big man, eh?

      Like

      • If they want virile leadership, they need to hire Berlusconi …..and Putin….any maybe some young attractive eastern european women to seal the deal.

        Like

  2. Cant Give

    You having been exposed to such an environment, I can see where your conviction (or rage) is coming from. You mention the lab you were trained in. How do you feel about that? Did you notice anything during your stay?

    Like

  3. J Biol Chem. 2005 Jan 14;280(2):1199-208. Epub 2004 Nov 8.
    A novel repressive E2F6 complex containing the polycomb group protein, EPC1, that interacts with EZH2 in a proliferation-specific manner.
    Attwooll C1, Oddi S, Cartwright P, Prosperini E, Agger K, Steensgaard P, Wagener C, Sardet C, Moroni MC, Helin K.
    Author information
    1
    European Institute of Oncology, Department of Experimental Oncology, Via Ripamonti 435, Milan, 20141, Italy.

    Figure 5B.

    Figure 6B, late G1.

    Figure 6B S phase.

    Like

    • Nucleic Acids Res. 2010 Aug;38(15):4958-69. doi: 10.1093/nar/gkq244. Epub 2010 Apr 12.
      https://pubpeer.com/publications/8C28E946204CBB403FC8E11CE92301

      Characterization of an antagonistic switch between histone H3 lysine 27 methylation and acetylation in the transcriptional regulation of Polycomb group target genes.
      Pasini D1, Malatesta M, Jung HR, Walfridsson J, Willer A, Olsson L, Skotte J, Wutz A, Porse B, Jensen ON, Helin K.
      Author information
      1
      Biotech Research and Innovation Centre (BRIC), University of Copenhagen, Copenhagen, Denmark.

      Figure 5C.

      Like

  4. Myriam Alcalay, presently director of Experimental Haematology Unit at IEO, pretended on PubPeer not to understand the concerns:

    “it is difficult to infer what they are pointing to with the boxes and arrows. […] The faint bands indicated by Petrocephalus and Pseudanapaea are clearly non-specific bands, also present in other lanes, that in no way interfere with the specific bands or alter the significance of the experiment. The result was also confirmed with a different technique (comet assay) as shown in figure 4c. “

    Another possibility is that she is thick.

    A nepotistic society means that the thick rise to the top.

    https://www.bbc.co.uk/news/magazine-21507168
    https://www.nature.com/news/italy-s-anti-nepotism-drive-picked-up-in-surname-study-1.22242

    Like

  5. Miscappalorina

    Making a summary: Di fiore, Pelicci Mantovani, Helin, Marine, Di Croce, Blasi all dishonest persons. On the other side leonid Schneider. I hope they will not even waste time reading your articles full of rage for having failed in your life.

    Like

    • Hey, Berlusconi did well also. But your comment shows the Italian attitude to any kind of ethics: it’s all about money and power, nothing else matters.
      hence, congrats on your past and present governments. Enjoy the elections, with Duce Salvini.

      Like

      • I’m Italian and I plaude to the action taken against these scientific frodes occurred in Italy.
        Regarding your sentence “…the Italian attitude to any kind of ethics…”, you should better take it back.
        60 million people cannot be grouped as you describe. You understand that, don’t you?
        So, take it back.

        Like

      • Mate, shall I tell you why no action is being taken against Ferrara rector Giorgio Zauli? Because of his Lega connections. If you now mean to say, Lega is not Italy, take a hike.
        And how about Macchiarini affair in Firenze? At least 5 DEAD, nobody cares, because Toscana Presidente Rossi is Macchiarini’s friend. That is Italy and its politics. Deal with it, literally.

        Like

  6. Reply to Miscappalorina August 16, 2019

    Di fiore, Pelicci Mantovani, Helin, Marine, Di Croce, Blasi all have not followed standard practice for publishing data. Is that of any importance to you?

    Like

  7. Rex Rictor

    @Miscappalorina why dont you crawl back in the dark you came from…Italy that is. When will your country ever grow up, its a disgrace to Europe and humanity. Yea you make good food but you sure as hell can’t do much else. Your science and ethics is a disgrace, how you treat young scientists is disgusting and deplorable. Plain and simple. You just made my day.

    Like

    • Its all relative. Hate to say it, but there is a lot of “science” being churned out of the People Republic of China that is wrong. My guess is that the Chinese (in the PRC), in general, are even more heirarchal than Italians, and therefore are probably less ethical (I admit this is speculation, based on what I see in the Chinese labs in the US). Probably more garbage papers per total paper ratio for China than Italy. This is not a race issue, but, dare I say, an incentive and cultural issue. At least Italy has basic western values of transparency and freedom of speech.

      Like

      • Reply to NMH August 16, 2019

        There might be something in what you say.

        In the PRC the trial is in the morning, the execution in the afternoon. No death penalty in Italy.
        That is an absolute difference.

        Political parties (note the plural) are allowed to exist in Italy. There is only one political party/management system in the PRC. That is quite a difference.

        People are allowed to vote for whichever party they want in Italy.

        “At least Italy has basic western values of transparency and freedom of speech.”

        https://www.transparency.org/country/ITA Rank 53/180
        https://www.transparency.org/country/CHN Rank 87/180

        Yes, Italy is more transparent than the PRC.

        But not so transparent as
        Germany https://www.transparency.org/country/DEU Rank 11/180
        or France https://www.transparency.org/country/FRA Rank 21/180

        When it comes to freedom of speech Italy does have Omertà, which some say has spread to the whole country since WWII.

        “Probably more garbage papers per total paper ratio for China than Italy”. I think that is worth looking into. I get a feeling that Elisabeth Bik may have published on that.

        It is not a reason for not looking at Italy, or anywhere for that matter. The most transparent countries will likely still have problematic data.

        Like

      • Don’t forget about cultural differences, in other words, what do the individual people value in particular culture? And are these “average” values different than in another culture (again, NOT race)?

        Do all people on the planet earth want to live “the good life”? Absolutely.

        But some individuals are more likely to be unethical to get this than others, and I think with some cultures the bell curve of this activity is shifted more toward unethical behavior, and this is also manifest in the behavior of the scientists doing research. Some cultures value prestige and money, and “getting ahead” more than others. Just my opinion.

        Like

    • I’m Italian and I indeed plaude to the action taken against these scientific frodes. Legal action has also been taken and the judiciary recognized PPDF and others guilty, although a period of detention cannot be imposed because a specific law is currently missing. That said, you Rex Bla Bla, by saying what you said about Italians, you just qualify yourself as a little person with no shred of intelligence and decency. You ought be in a cage.

      Like

  8. My memories of Di Fiore from an EMBO conference in Croatia many years ago is a man burping when he present his data. When we left the meeting, he and his family were sitting in the seat in front of us at the first class zone enjoying champagne and still burping.

    I realized some years ago that the time spent reading his papers was wasted.

    Like

  9. Reply to MMH August 16, 2019

    “But some individuals are more likely to be unethical to get this than others, and I think with some cultures the bell curve of this activity is shifted more toward unethical behavior, and this is also manifest in the behavior of the scientists doing research.”

    I agree. Why should “scientists” be any different than their society?

    Like

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  11. “Another mentee of Di Fiore is IFOM group leader Giorgio Scita”

    Cell Death Dis. 2014 Jan 16;5:e1005. doi: 10.1038/cddis.2013.465.
    Osteolytic bone metastasis is hampered by impinging on the interplay among autophagy, anoikis and ossification.
    Maroni P1, Bendinelli P2, Matteucci E2, Locatelli A3, Nakamura T4, Scita G5, Desiderio MA2.
    Author information
    1
    Istituto Ortopedico Galeazzi, IRCCS, Milan, Italy.
    2
    Dipartimento di Scienze Biomediche per la Salute, Molecular Pathology Laboratory, Università degli Studi di Milano, Milan, Italy.
    3
    1] Dipartimento di Scienze Biomediche per la Salute, Molecular Pathology Laboratory, Università degli Studi di Milano, Milan, Italy [2] Istituto FIRC di Oncologia Molecolare, Milan, Italy.
    4
    Center for Advanced Science and Innovation, Osaka University, Yamadaoka 2-1, Osaka 565-0871, Japan.
    5
    1] Istituto FIRC di Oncologia Molecolare, Milan, Italy [2] Dipartimento di Scienze della Salute, Università degli Studi di Milano, Milan, Italy.

    https://pubpeer.com/publications/A4E0B695593437C47F947DFE5BDCD7

    Like

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  13. “Molecular Biology of the Cell (2004) doi: 10.1091/mbc.e03-06-0427”
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC307530/
    The eps8 Family of Proteins Links Growth Factor Stimulation to Actin Reorganization Generating Functional Redundancy in the Ras/Rac Pathway
    Nina Offenhäuser,† Alessandro Borgonovo,† Andrea Disanza,† Pascale Romano,* Isabella Ponzanelli,† Gioacchin Iannolo,† Pier Paolo Di Fiore,†‡ and Giorgio Scita*†§

    2019 correction
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743360/

    The authors of “The eps8 Family of Proteins Links Growth Factor Stimulation to Actin Reorganization Generating Functional Redundancy in the Ras/Rac Pathway” (Mol. Biol. Cell [2004] 15, 91–98; originally published online as 10.1091/mbc.E03-06-0427) wish to make a correction to Figure 4B of the article.

    It has been brought to our attention that in the original HTML and PDF versions, during the assembly of Figure 4B, we made the following errors during the assembly of the final Figure 4B from the original autoradiograms and nitrocellulose membranes:

    a) We erroneously flipped by 180° the top rightmost blot; and

    b) we inadvertently duplicated the rightmost blot, and duplicated and erroneously trimmed the bottom leftmost.

    We apologize for these errors and oversights. The corrected Figure 4 is shown below. These corrections do not change the interpretation of the published experiments.

    Like

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