Academic Publishing Research integrity

Journals deny corrections to Chiara Francavilla

Manchester scientist Chiara Francavilla discovered duplicated images in her older papers and submitted correction requests to Molecular Cell and JCB, only to be ignored.

A nightmare for every scientist: a mistake is found in your published paper, like some duplicated images, and you ask the journal for corrections. Yet the editors do nothing and ignore your pleas. This is what apparently happened to the young group leader at the University of Manchester, the cell biologist Chiara Francavilla. The two papers affected are from her PhD thesis in Milan, Italy, and postdoctoral studies in Copenhagen, Denmark. As soon as she found out about the duplicated microscopy images, Francavilla submitted correction requests to the two journals in question, Molecular Cell and Journal of Cell Biology (JCB). It is unsurprising that the former, an Elsevier outlet, ignored all requests for correction, this is unfortunately the known attitude at Cell on data integrity. But with JCB, a society journal published by Rockefeller University Press, this behaviour is most unexpected. JCB used to be the trailblazer in research integrity, first to employ image integrity analysis on all accepted manuscripts. It started with the seminal 2004 article by the editors Mike Rossner and Kenneth Yamada: “What’s in a picture? The temptation of image manipulation”. That editorial is cited basically on every occasion and in every paper when image data integrity is being discussed. What happened since then?

First, the Molecular Cell paper, which contains several duplicated microscopy images.

Chiara Francavilla, Kristoffer T.G. Rigbolt, Kristina B. Emdal, Gianni Carraro, Erik Vernet, Dorte B.Bekker-Jensen, Werner Streicher, Mats Wikström, Michael Sundström, Saverio Bellusci, Ugo Cavallaro, Blagoy Blagoev, Jesper V. Olsen

Functional Proteomics Defines the Molecular Switch Underlying FGF Receptor Trafficking and Cellular Outputs

DOI: 10.1016/j.molcel.2013.08.002  26 September 2013

Mol Cell Fig 1

This Figure 1 contains 3 duplicated images, highlighted with colour frames. Those are not exact duplications, all three images are shifted against each other, in particular the red and yellow marked ones. This is likely because in all cases a large confocal microscopy image was inadvertently re-used as two different areas of interests were selected to show two different experiments at two different time points, and with two different settings for signal intensity.

Another figure in that paper also contains three duplications:

Mol Cell Fig S5

That Molecular Cell study was performed at the lab of Jesper Olsen, professor at University of Copenhagen and group leader at the associated Novo Nordisk Foundation Center for Protein Research. The other paper was done at IFOM (the FIRC Institute of Molecular Oncology) in Milan (see my conflict of interest statement at the end),  it was published in JCB in 2009:

Chiara Francavilla, Paola Cattaneo, Vladimir Berezin, Elisabeth Bock, Diletta Ami, Ario de Marco, Gerhard Christofori, Ugo Cavallaro
The binding of NCAM to FGFR1 induces a specific cellular response mediated by receptor trafficking
DOI: 10.1083/jcb.200903030 | Published December 28, 2009

The issue here is one duplicated confocal microscopy image, highlighted with red frame. JCB Fig6

The two images (SU6656, 30′) show differently stimulated cells, and are identical except that the green signal for HA-FGFR1 is for some reason somewhat stronger in the FGL image compared to the FGF-2 one. The blue DNA signal is however of similar intensity in both images. It is probably also an oversight, as the last author and Francavilla’s former PhD advisor Ugo Cavalaro explained to me:

“Chiara knows about these issues on both papers and she informed me as well. I do not have much control on the Mol Cell paper (those data have been generated in Copenhagen), but I know Chiara is actively working to solve every single issue.
As soon as I learnt about the image duplication in our JCB paper (slide 4 in your file), I retrieved the original data and found out that it was a simple mistake (as you indicated, the same image was used twice). Next, I immediately contacted JCB and provided the correct version of the figure. JCB editor (Timothy Spencer) offered to publish a correction, for which we provided an accompanying statement. All this happened between June 27 and July 2, 2018 (I can provide the mail exchange if you wish). I didn’t check whether the correction has been published already, if not it should be published soon.
So JCB is aware of the mistake, and I guess Chiara and Jesper Olsen have informed Mol Cell as well.”

Cavallaro did provide me with his email exchange with JCB editor Tim Spencer from summer 2018. Cavallaro and Francavilla requested a correction, and explained the image duplication in Figure 6:

“Our mistake has no impact on the conclusion from the experiment. In fact, both conditions gave negligible green fluorescence staining without acid wash, meaning that no or very little localization of HA-FGFR1 on the cell surface was detected upon treatment with either FGF2 or FGL for 30 minutes (or 120) and Src inhibition with SU6656. Of note, this confirmed the results shown in the panels above where the other Src inhibitor PP1 was used.”

Spencer agreed with issuing a correction:

“We agree that a correction should be issued and the offending image replaced.
Please provide us with a short statement which explains the error to the journal’s audience. This text should indicate the error (you don’t need to indicate how the duplication occurred, just that the image used in the FGL treatment condi-on was incorrect), what was done to fix the error, and how this changes interpretation of the conclusions (if at all). Once you send us that text, we can proceed with issuing the correction.”

On same day, 2 July 2018, Cavallaro sent Spencer and JCB this correction text:

“The authors discovered after publication that one of the images used in Fig. 6A, namely the image related to the 30-minute treatment with FGL in the presence of SU6656 (in the “- acid wash” condition), was incorrect. That image has now been replaced with the correct one, corresponding to that treatment condition. This correction does not change in any way the conclusion from the experiment, namely that no or very little localization of HA-FGFR1 on the cell surface was detected upon treatment with either FGF2 or FGL upon Src inhibition. The authors regret any confusion this may have caused.”

And yet, neither by Molecular Cell (who were apparently also contacted by authors), or by JCB, was any correction ever issued. As mentioned above, one is not surprised with Cell journals, they reject not only corrections requests but also official institutional demands for retractions because Cell editors know it better (read here, here and here).  But why JCB?

Mike Rossner lost his job with JCB when Rockefeller University Press was restructuring in 2013, maybe this was when the policies on image integrity changed?  The journal and its new Editor-in-Chief refused all communication with me, unfortunately.

Cavalaro is not directly associated with IFOM anymore, but with IEO (European Institute of Oncology) and he still works at the same densely packed IFOM-IEO campus in Milan. One of the 10 Scientific Editors at JCB is Pier Paolo Di Fiore, former scientific director of IFOM, now IEO-affiliated and at all times one of the most senior scientists at the IFOM-IEO campus. Cavallaro is presently member of Di Fiore’s research team there. It is probably all a coincidence, just like the image duplications in Francavilla’s papers.

There is not much hope to get research institutions and universities to exert pressure on the journals to finally allow corrections of the literature. Both the University of Copenhagen and University of Manchester had their own cases of truly problematic research, yet even there they did very little to nothing. Manchester proudly employs the notorious zombie scientist Silvia Bulfone-Paus at their school of Biological Sciences (where Francavilla also works). Bulfone-Paus renounced her position as institute director in Germany after 12 retractions (story here), but despite everything she is certainly not affected by the mass-redundancy plans for Manchester University professors.

And at Copenhagen, the case of Janine Erler remains unresolved, no papers were corrected or retracted, but the whistleblowers met with an unfortunate accident when all their cell lines were accidentally thawed and destroyed (read here).

Update: just hours after this article was published, I received this email from JCB Executive Editor Rebecca Alvania:

We examined the materials Dr. Francavilla provided in July 2018 to correct her 2009 JCB paper and approved the correction. Unfortunately, due to a miscommunication on our end the correction files failed to reach our production staff. We are sending the files to production and the correction will be published as soon as possible. Thank you for alerting us to this, we appreciate the opportunity to ensure the published record is accurate. 

Cavallaro received this message from Alvania:

“I want to apologize for our error in failing to publish the below correction in a timely manner. Although the materials were approved editorially at the time, due to a miscommunication on our end the correction failed to reach our production staff. We are sending the materials to production now and the correction will be published as soon as possible.”

And what will Molecular Cell do?

Conflict of interest statement: I worked as postdoc in 2008-2012 at IFOM and the IFOM-IEO campus, and know Francavilla, Cavallaro and Di Fiore in person. I never had any significant interactions or conflicts with any of them, then or since.


 

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26 comments on “Journals deny corrections to Chiara Francavilla

  1. J Clin Invest. 2003 Dec;112(11):1751-61.
    Acute myeloid leukemia fusion proteins deregulate genes involved in stem cell maintenance and DNA repair.
    Alcalay M1, Meani N, Gelmetti V, Fantozzi A, Fagioli M, Orleth A, Riganelli D, Sebastiani C, Cappelli E, Casciari C, Sciurpi MT, Mariano AR, Minardi SP, Luzi L, Muller H, Di Fiore PP, Frosina G, Pelicci PG.
    Author information
    1
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy.

    Figure 4e.

    Like

    • Pier Giuseppe Pelicci is another of the 3 giants running the IFOM-IEO Campus, incidentally both his wife and his sister are group leaders there. Third Campus giant is Marco Foiani, currently IFOM director.
      Myriam Alcalay is now group leader at IEO, at same IFOM-IEO Campus.

      Like

  2. Rix Rector

    Probably just another co-incidence Kristian Helin is former IFOM. He is also former boss for Janine Erler at KU.

    https://www.ifom.eu/en/cancer-research/publications/2006.php

    Like

  3. “University of Manchester ha[s its] their own cases of truly problematic research”.

    Associate Dean of Business Engagement, co-Director of Manchester Centre for Cellular Metabolism, Theme lead for Personalised Therapy and Professor of Molecular Cell Biology, Division of Medical and Clinical Cancer Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Sciences Centre, University of Manchester.

    https://www.research.manchester.ac.uk/portal/paul.townsend.html

    “After reading for my doctorate at the Imperial Cancer Research Fund (now CRUK) in London (1997), I undertook post-doctoral research at Imperial College London, Southampton and University College, London”

    Retractions:-

    First author https://pubpeer.com/publications/0246C9C598B9CA1EC447D027477C1E

    https://pubpeer.com/publications/62BA2A5FE6003D1518BA264289A406

    https://pubpeer.com/publications/FE8E2C05300A31AFD0E034A72C1635

    https://pubpeer.com/publications/0AA52F5AAFA10FE9AB461A7A69C64B

    Problematic data:-

    https://pubpeer.com/publications/DA1732355927FD9DAC97B8644891DC

    https://pubpeer.com/publications/A5BFA629332D2BA85AA6AD72E88FDF

    https://pubpeer.com/publications/D075F9AE6DE4D5D26F46C8D727377D

    https://pubpeer.com/publications/4D4CAE2CC16006D3E149A9821F44B2

    https://pubpeer.com/publications/056724744849CEC288887B40A7EFE1

    https://pubpeer.com/publications/7488A57682BDE767B66F9DF941211D

    https://pubpeer.com/publications/75ACD214E365043EDC1FAC58BB782C

    https://pubpeer.com/publications/0AC7CCD94F60BC8264304830524827

    https://pubpeer.com/publications/2AC9EFB5CE280B9BAC8FED2F2C33CA

    https://pubpeer.com/publications/18E46EC4C6F3AE869A700DAC133838

    https://pubpeer.com/publications/EFC648A4F0C46BCA8F715BFA8EB89F

    https://pubpeer.com/publications/33DA1C016505DD64C089C9F2E699FD

    https://pubpeer.com/publications/3000EBE3FE90E3A056CEB81417C09B

    https://pubpeer.com/publications/AE3AE281886A278786FD30D14CEEE6

    See: https://retractionwatch.com/2018/03/19/clear-signs-of-manipulation-in-paper-co-authored-by-prominent-geneticist/

    4th and 5th retractions in comments section.

    Like

  4. “IEO (European Institute of Oncology)”

    Mol Cell Biol. 2002 Oct;22(20):7351-63.
    The neuron-specific Rai (ShcC) adaptor protein inhibits apoptosis by coupling Ret to the phosphatidylinositol 3-kinase/Akt signaling pathway.
    Pelicci G1, Troglio F, Bodini A, Melillo RM, Pettirossi V, Coda L, De Giuseppe A, Santoro M, Pelicci PG.
    Author information
    1
    Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy.

    https://pubpeer.com/publications/A4AE3813ADDAFF690C1100D58C4C92#2

    Science. 2002 Feb 8;295(5557):1079-82.
    Methyltransferase recruitment and DNA hypermethylation of target promoters by an oncogenic transcription factor.
    Di Croce L1, Raker VA, Corsaro M, Fazi F, Fanelli M, Faretta M, Fuks F, Lo Coco F, Kouzarides T, Nervi C, Minucci S, Pelicci PG.
    Author information
    1
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy.

    https://pubpeer.com/publications/EE38DB6A453E712026F48E4AB9343C

    Aging Cell. 2013 Jun;12(3):435-45. doi: 10.1111/acel.12060. Epub 2013 Mar 27.
    Oxidative stress activates a specific p53 transcriptional response that regulates cellular senescence and aging.
    Gambino V1, De Michele G, Venezia O, Migliaccio P, Dall’Olio V, Bernard L, Minardi SP, Della Fazia MA, Bartoli D, Servillo G, Alcalay M, Luzi L, Giorgio M, Scrable H, Pelicci PG, Migliaccio E.
    Author information
    1
    European Institute of Oncology, Via Ripamonti 435, Milan, 20141, Italy.

    https://pubpeer.com/publications/5B5EF2E4B2C92B80EEF1D6A3C9E212

    Like

  5. Milano is a scientific wonderment. Molecular correction of muscularr dystrophy in mice in 2000!

    Hum Mol Genet. 2000 Jul 22;9(12):1843-52.
    Transplacental injection of somite-derived cells in mdx mouse embryos for the correction of dystrophin deficiency.
    Torrente Y1, D’Angelo MG, Li Z, Del Bo R, Corti S, Mericskay M, DeLiso A, Fassati A, Paulin D, Comi GP, Scarlato G, Bresolin N.
    Author information
    1
    IRCCS Ospedale Maggiore Policlinico, Milan, Italy.

    https://pubpeer.com/publications/E6A3CA7A0BE840CBF7308779D97601

    Figure 4.

    Like

  6. Pingback: "Vaccinazismo" - Ocasapiens - Blog - Repubblica.it

  7. Pingback: Manchester: “research misconduct concerned only one member of the research group” – For Better Science

  8. Nature. 2002 Mar 28;416(6879):451-5.
    A single motif responsible for ubiquitin recognition and monoubiquitination in endocytic proteins.
    Polo S1, Sigismund S, Faretta M, Guidi M, Capua MR, Bossi G, Chen H, De Camilli P, Di Fiore PP.
    Author information
    1
    Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141, Milan, Italy.

    Figure 2b

    Like

  9. Nature. 1999 Sep 16;401(6750):290-3.
    EPS8 and E3B1 transduce signals from Ras to Rac.
    Scita G1, Nordstrom J, Carbone R, Tenca P, Giardina G, Gutkind S, Bjarnegård M, Betsholtz C, Di Fiore PP.
    Author information
    1
    Department of Experimental Oncology, European Institute of Oncology, Milan, Italy.

    Figure 3a

    For such a prestigious journal the image resolution is very low. Has added advantage for Nature as provides a defense for their best paying customers.

    Like

  10. Mol Cell Biol. 2004 Mar;24(6):2526-35.
    Np95 is a histone-binding protein endowed with ubiquitin ligase activity.
    Citterio E1, Papait R, Nicassio F, Vecchi M, Gomiero P, Mantovani R, Di Fiore PP, Bonapace IM.
    Author information
    1
    Istituto FIRC di Oncologia Molecolare, 20139 Milan, Italy.

    High resolution figure 3B.

    Mol Cell Biol does give a toss as it is before their self-declared 6 year limit, and saves then from having to do any work. Laziness damages science. Something Mol Cell Biol should be proud of.

    https://retractionwatch.com/2017/06/09/journal-wont-look-allegations-papers-six-years-old-public-websites/

    Like

  11. Mol Biol Cell. 2004 Jan;15(1):91-8. Epub 2003 Oct 17.
    The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway.
    Offenhäuser N1, Borgonovo A, Disanza A, Romano P, Ponzanelli I, Iannolo G, Di Fiore PP, Scita G.
    Author information
    1
    The FIRC Institute for Molecular Oncology, 20134 Milan, Italy.

    https://pubpeer.com/publications/71390C0A3A36501ED4789882B3BCFD#2

    Figure 4B.

    Has Mol Biol Cell ever corrected/retracted a paper?

    Like

  12. Pingback: Pier Paolo and Pier Giuseppe, the titans of IFOM-IEO – For Better Science

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