In Spain, there seems to be a tradition of reacting to emerging evidence of data manipulation with handing out prestigious awards to authors of these papers. This is how we were all taught that all the PubPeer evidence matters nothing whatsoever, as Pura Munoz-Canoves, Maria Pia Cosma and Manel Esteller were celebrated with prizes and grants for their research achievements (read here , here and here). Now there is a new popular academic hero in Spain, Carlos López-Otín, Professor of Biochemistry and Molecular Biology at the University of Oviedo, and EMBO member. Lopez-Otin was now awarded a 2017 Mentoring Award, presented to him by Sir Philip Campbell, on behalf of his journal Nature, which Editor-in-Chief Sir Philip has been for the last 22 years.
The journal Nature remains the highest authority in science, this Mentoring Award is Sir Philip’s parting present to the Nature-reading scientific community, as he is being promoted inside Springer Nature’s publishing hierarchy. It is in this way a statement of what Nature and its publisher think of research integrity. Lopez-Otin’s evidence record on PubPeer is telling enough, however most of the evidence of digitally manipulated gel images there was posted around 2-3 months ago, by the pseudonymous Claire Francis. Maybe the evidence appeared too late, and the award decision couldn’t be stopped anymore. Which would explain why Nature deleted their own tweet announcing the mentorship awards after I replied to it with PubPeer evidence on Lopez-Otin’s papers. According to another Nature website, which was for some reason also deleted, the closing date for nominations was Monday 31 July 2017. So I share some examples below, all are from papers where Lopez-Otin is last and corresponding author.
Nature tweeted the article about mentorship awards in Spain, to which I replied. Unlike with other social media, you cannot remove replies to your tweets if you disagree with those. Pop, the original Nature tweet was deleted, Nature followers on Twitter were then spared reading this filth:
The Nature site which invited award nomination from Spain, was gone as well. This was the awarding committee, according to residues that site left Google search and this source:
Chair of the judges: Josep Penuelas, Center for Ecological Research and Forestry Applications (CREAF) – National Research Council (CSIC), Barcelona
Judging panel:
Alison Abbott, Nature, Munich, Germany
Emilia R. Solano, CIEMAT, Madrid, Spain
Juan Lerma, Instituto de Neurociencias de Alicante – UMH, Alicante, Spain
Mariano Barbacid, Centro Nacional de Investigaciones Oncológicas, Madrid, Spain
Pilar Ruiz Lapuente, Institute of Cosmos Sciences, University of Barcelona, Barcelona, Spain
For some reason, jury member Alison Abbot failed to mention her decisive role in her follow-up Nature article, while the past one listing her as member of the judging panel was deleted.
When issuing the award, Sir Philip said about Lopez-Otin:
“Spain’s great examples are no exception – they are extraordinary in their ability to nurture emerging scientists of great diversity.”
Extraordinary re-use of a loading control indeed, in this paper from Lopez-Otin lab:
Alison Abbot, who was one of the judges, later wrote in this Nature article:
“López-Otín’s mentees praised his ability to draw out and encourage even the shyest lab member. “Carlos gave me the strength to pursue an independent career as a principal investigator,” one wrote. “He made me feel that my career was a priority for him, and it is thanks to him that my career has advanced further than I would ever have imagined.” Another noted how López-Otín had encouraged trainees to read widely and think big. “Carlos told me that ground-breaking work comes from broad scientific knowledge, while overspecialization is the path to small science.”
Nominators said that López-Otín insisted on celebrating every accepted paper or project in the lab and was widely generous with advice”.
One wonders, how was this copy-pasting on the background, apparently required in order to get this paper accepted, celebrated?
Another quote from the Nature article by Alison Abbot:
“Carlos has been a constant catalyst of scientific activities in Asturias, a region marked by decades of economic and social distress,” wrote one of his mentees”.
In this vein, was prompting people to clone gel bands part of those catalytic activities of the mentorship award-winner Lopez-Otin?
Even Sir Philip’s own Nature family wasn’t spared of Lopez-Otis irregularities. This PubPeer post discusses Soria-Valles et al, Nature Cell Biology, 2015, and how the provided original blot images in the supplementary data do not match their published figure counterparts.
Donate!
If you are interested to support my work, you can leave here a small tip of $5. Or several of small tips, just increase the amount as you like (2x=€10; 5x=€25). Your generous patronage of my journalism will be most appreciated!
€5.00
For Better Science 
For sure artistic creativity must be compensated….indeed a good part of a PhD in Spain is dedicated how to do the best Adobe Photoshop artistic jobs….
LikeLike
Well Ana,
Sounds like you speak from experience, as, to the best of my knowldege, you are supporting such an UNTRUE statement by no evidence appart from your own PhD (and perhaps someone else’s in your environment?) in Spain.
Bottom line: you are just fabricating the data on which you based your conclusions. Therefore, in the light of your affirmation, I could probably say that you did a great PhD in Spain, right?. Good for you then!!
LikeLike
Dear Manuel
Indeed I did a great, fair and clean PhD in Spain and I am truly thankful to a couple of Spanish who helped me in their painful crusade against their own system
Everybody knows about how the system works in Spain and most of the academics are afraid of talking fearing represalies
In Portugal the system is similar, fortunately we have an research fellows association who intends to defend their rights, ABIC with many difficulties
LikeLike
Dear Ana,
I cannot do other thing that support Manuel and feel sorry for the pathetic environment you had on your PhD, mine, also in Spain, was full of amazing scientists, that were able to publish in very good journal with much less support than other countries. I learnt very good science, how to be a good scientist, and testimony of that is the good job I am doing outside Spain. I’m super proud, and it was here in UK the first time I faced lies in science and had to live with it. You are an arrogant and an ignorant by thinking that you were the only honest scientist in Spain or one of the “few”…keep doing that thinking you are the best, that kind of humility and respect to others is exactly what a good scientist should be…
LikeLike
Yes, they should replicate the Portuguese system.
LikeLike
Pingback: Boletim de Notícias: especialista defende cálculo de R$ 1 trilhão para MP do petróleo | Direto da Ciência
You have to congratulate Nature on bringing the data manipulation to public attention.
Nature may be unwitting, but it does have high impact.
LikeLike
In reply to Isabel January 10, 2018 at 18:05
“Spain, was full of amazing scientists”
Care to name a few?
LikeLike
With that question you don’t even deserve an answer…
LikeLike
Dear Isabel,
I am still waiting you to name 20 “amazing scientists” in Spain.
It should not be difficult as you wrote that “Spain, was full of amazing scientists”.
If you cannot name 20 where have they gone?
LikeLike
I hope we could end this debate by agreeing there are in fact quite a lot of very good scientists in Spain, but under current conditions they must live in fear of their dishonest peers who accumulate power, grants and awards. An award given to these cheaters is not just a meaningless cup or a medal. It is a sign who enjoys protection from the very top. Spain as a former oppressive dictatorship surely knows how this works.
LikeLike
“Spain as a former oppressive dictatorship surely knows how this works.” If I had any interest in this blog, this kind of bigoted and scientific statement unveils who you really are.
LikeLike
No, you wrote that “Spain was full, of amazing scientists”. Name 20.
LikeLike
You know there are 20 and more. Yes, those that you mention are not part of that group. What else do you need?
What is your plan to improve the scientific environment in Spain? Write helpful stuff that can be applied, hopefully without prejudice and racism. Don’t let your anger take the best of you.
LikeLike
Dear Angela,
Many feel that scrutiny is not nice.
LikeLike
Yolanda Revilla.
http://www.cbm.uam.es/joomla-rl/index.php/en/research/scientific-departments/virology-and-microbiology?id=691
https://pubpeer.com/publications/D5D1F1DCC207C82E0888E921627AA5
https://pubpeer.com/publications/86A3C0F9F2D138C79192A527730EEA
https://pubpeer.com/publications/3F53F9301766DAA28FA092AC1832CC
https://pubpeer.com/publications/C6177B50409C1846E07202A052F590
https://pubpeer.com/publications/6BDB25D8179779C2F176CF22776952
https://pubpeer.com/publications/468C9F2FEC0C12762A4469269BC6C7
LikeLike
Maria Gasset.
Instituto Química-Física Rocasolano, Consejo Superior de Investigaciones Científicas, Serrano 119, 28006 Madrid, Spain.
https://pubpeer.com/search?q=gasset
LikeLike
Manuel Benito.
https://www.ucm.es/biomol2/directorio?id=2663
https://pubpeer.com/search?q=manuel+benito
http://retractionwatch.com/2017/04/25/harvard-diabetes-researcher-retracts-third-paper/
LikeLike
Mireia Dunach.
http://uab.academia.edu/MireiaDuñach
https://pubpeer.com/search?q=+dunach
LikeLike
Antonio Celada.
https://www.researchgate.net/profile/Antonio_Celada
https://pubpeer.com/search?q=celada
LikeLike
Susana Gonzalez.
http://retractionwatch.com/2017/09/18/stem-cell-scientist-appealing-dismissal-loses-another-paper/
http://retractionwatch.com/2016/03/07/stem-cell-researcher-in-spain-dismissed-following-investigation/
https://elpais.com/elpais/2016/03/04/ciencia/1457090679_248492.html
LikeLike
Jose M Rojas.
http://www.isciii.es/ISCIII/es/contenidos/fd-el-instituto/fd-organizacion/fd-estructura-directiva/fd-subdireccion-general-servicios-aplicados-formacion-investigacion/fd-centros-unidades/fd-unidad-funcional-de-investigacion-de-enfermedades-cronicas/fd-areas-y-unidades-UFIEC/unidad-de-biologia-celular.shtml
https://pubpeer.com/publications/89582C2164A1D9F4764540B7A83E2B
https://pubpeer.com/publications/C20FE9AAE2120DFC44CFCB7DFDB6F2
https://pubpeer.com/publications/F0E03F9AD1B066658E1605341A3D4E
https://pubpeer.com/publications/E37EB191FB71797127BF586EBDCC11
https://pubpeer.com/publications/D6ABBDA2D87FF215C2083F2C9EC576
https://pubpeer.com/publications/A18472472618ADF24C65BD6BF010DC
https://pubpeer.com/publications/86B96C3D8A46010B7DF4E7D25CB4DD
https://pubpeer.com/publications/36548E08E70595A39B3D2A79BBB852
LikeLike
Margarita Lorenzo.
Dead, but they should clear up the problematic data,
https://elpais.com/diario/2010/04/17/necrologicas/1271455201_850215.html
and not do virtue signalling
https://www.fundacionlilly.com/es/actividades/premios-fundacion-lilly/premio-margarita-lorenzo.aspx
https://pubpeer.com/search?q=margarita+lorenzo
LikeLike
Felix Bonilla.
https://www.pubfacts.com/author/Felix+Bonilla
Hospital Universitario Puerta de Hierro
https://pubpeer.com/search?q=felix+bonilla
LikeLike
Antonio Garcia de Herreros.
https://www.imim.cat/programesrecerca/cancer/en_ubcm.html
https://pubpeer.com/publications/BDEB1BEC0F27AC6D5AFDD0D0E980F7
https://pubpeer.com/publications/D9AB005FB5B07483BC12C2C0DFB454
https://pubpeer.com/publications/5A785B0CF23403E24C25D3C43111FB
https://pubpeer.com/publications/981BDA5DB6DFD80DCC41AEF1808BE9
https://pubpeer.com/publications/BEB69B48326037191631A1B13D607C
https://pubpeer.com/publications/4166CB88F57104A834DDEF71515EEC
https://pubpeer.com/publications/A8F3E69DACCF638411E66257F47A9F
https://pubpeer.com/publications/447EC5BFD11D3D1D947100DAB271F9
https://pubpeer.com/publications/2EB431B880F997FBCDB5A4E48927FD
https://pubpeer.com/publications/034CC3FA793059AD615B5A5D80BD84
https://pubpeer.com/publications/95D7EDC45C43706AB46E45AE03F1A1
https://pubpeer.com/publications/7A2D9BBDCE85623CB4824CA04077E0
https://pubpeer.com/publications/07643B5654697153BE6504D2C3F966
https://pubpeer.com/publications/2F0AF28466D2E1D31510ACC72794F1
https://pubpeer.com/publications/8A9B324060E1673639C5114CC0F3C5
https://pubpeer.com/publications/77ACF5E0CB3F2BB5DEAF2019B0CCE3
https://pubpeer.com/publications/C82AD606E1FE4DE152DA1D1796DE7B
https://pubpeer.com/publications/AE72300768ECE0EA987B4842EB30CA
https://pubpeer.com/publications/0E155718533A0156BBEAD8E8D59A0C
https://pubpeer.com/publications/0A32B29DEA2D2B0A10F4F362F55CD4
https://pubpeer.com/publications/E2D2996937DCDDAF9559725FDFDC95
https://pubpeer.com/publications/0E99B1B3B43B7D6917347AAA23EE7B
https://pubpeer.com/publications/42E81CC060E1BEA09DB2F7AAC9E484
https://pubpeer.com/publications/A62C2A767B8A9A60229DD116BCBB0D
https://pubpeer.com/publications/03C59A9C6383C9E70C9EEA7E056336
https://pubpeer.com/publications/DD8A003880FB4B4E2CE5218FD971D8
https://pubpeer.com/publications/7B8A5F590BF1468283CC3FE7D6BBCA
https://pubpeer.com/publications/E3471375942FBF3B5CBA8C3AC0C551
https://pubpeer.com/publications/7D64B99471B2AE86604500FC8D6569
LikeLike
J Biol Chem. 2004 Jun 18;279(25):26627-34. Epub 2004 Apr 15.
Identification and characterization of human and mouse ovastacin: a novel metalloproteinase similar to hatching enzymes from arthropods, birds, amphibians, and fish.
Quesada V1, Sánchez LM, Alvarez J, López-Otín C.
Author information
1
Departamento de Bioquimica y Biologia Molecular and Morfologia y Biologia Celular, Facultad de Medicina, Instituto Universitario de Oncologia, Universidad de Oviedo, 33006-Oviedo, Spain.
Figure 4. https://imgur.com/ZzWbrk5
Figure 3, see: https://pubpeer.com/publications/A2392B5A5FD19456E8AD74A68FCF80
LikeLike
NF-κB activation impairs somatic cell reprogramming in ageing
Nature Cell Biology (2015) – 4 Comments
doi: 10.1038/ncb3207 issn: 1465-7392 issn: 1476-4679 pubmed: 26214134
Clara Soria-Valles , Fernando G. Osorio , Ana Gutiérrez-Fernández , Alejandro De Los Angeles , Clara Bueno , Pablo Menéndez , José I. Martín-Subero , George Q. Daley , José M. P. Freije , Carlos López-Otín
https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18
Figure 2, 6, 7.
https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18#1
Figure 3.
https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18#3
Figure 7.
https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18#2
LikeLike
Still arguments continue on the paper of Soria-Valles et al…
NF-κB activation impairs somatic cell reprogramming in ageing
Nature Cell Biology (2015) – 4 Comments
doi: 10.1038/ncb3207 issn: 1465-7392 issn: 1476-4679 pubmed: 26214134
Clara Soria-Valles , Fernando G. Osorio , Ana Gutiérrez-Fernández , Alejandro De Los Angeles , Clara Bueno , Pablo Menéndez , José I. Martín-Subero , George Q. Daley , José M. P. Freije , Carlos López-Otín
https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18#10
Figure 6
Supplementary figure 6
https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18#9
Supplementary figure 8
https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18#5
LikeLike
Similarities between three Lopez-Otin papers:-
Cancer Res. 2000 Feb 15;60(4):877-82.
Human MT6-matrix metalloproteinase: identification, progelatinase A activation, and expression in brain tumors.
Velasco G1, Cal S, Merlos-Suárez A, Ferrando AA, Alvarez S, Nakano A, Arribas J, López-Otín C.
Author information
Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina, Universidad de Oviedo, Spain.
J Biol Chem. 2002 Oct 4;277(40):37637-46. Epub 2002 Jul 30.
Matriptase-2, a membrane-bound mosaic serine proteinase predominantly expressed in human liver and showing degrading activity against extracellular matrix proteins.
Velasco G1, Cal S, Quesada V, Sánchez LM, López-Otín C.
Author information
Departamento de Bioquimica y Biologia Molecular, Instituto Universitario de Oncologia, Universidad de Oviedo, 33006 Oviedo, Spain.
J Biol Chem. 2003 Feb 7;278(6):3671-8. Epub 2002 Nov 21.
Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy.
Mariño G1, Uría JA, Puente XS, Quesada V, Bordallo J, López-Otín C.
Author information
Departamento de Bioquimíca y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncología, Universidad de Oviedo, Spain.
See: https://i.imgur.com/PEdywou.png
LikeLike
J Biol Chem. 2005 Jan 21;280(3):1953-61. Epub 2004 Nov 9.
Human polyserase-2, a novel enzyme with three tandem serine protease domains in a single polypeptide chain.
Cal S1, Quesada V, Llamazares M, Díaz-Perales A, Garabaya C, López-Otín C.
Author information
1
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncología, Universidad de Oviedo, 33006 Oviedo, Spain.
Sample order differences between other papers and this one outlined in orange.
There are examples of re-use with sample order same.
See: https://imgur.com/D25yKrt
LikeLike
http://www.primaciatv.es/actor-antonio-banderas-bioquimico-carlos-lopez-otin-abriran-la-ceremonia-los-premios-fundacion-princesa-girona/
“El actor Antonio Banderas y el bioquímico Carlos López-Otín abrirán la ceremonia de los Premios Fundación Princesa de Girona”.
You have to give credit to Carlos López-Otín, he is also an “actor”.
LikeLike
J Biol Chem. 1999 Feb 19;274(8):4570-6.
Cloning and characterization of human MMP-23, a new matrix metalloproteinase predominantly expressed in reproductive tissues and lacking conserved domains in other family members.
Velasco G1, Pendás AM, Fueyo A, Knäuper V, Murphy G, López-Otín C.
Author information
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain.
See: https://i.imgur.com/iQugL8A.png
LikeLike
I had thought that “Wandering Western Blot Syndrome” was confined to Brazilian diabetes researchers, but evidently it is spreading!
https://pubpeer.com/publications/CD3325D6086BF005D1C72E75ED722D#11
LikeLike
Treaty of Tordesillas divided the newly “discovered” lands outside Europe between the Portuguese Empire and the Crown of Castile (present day Spain), along a meridian 370 leagues west of the Cape Verde islands, off the west coast of Africa. In effect, Portugal colonized what is now Brazil, Spain colonized most of the rest of South America. In this case we are seeing Spain. The spreading goes from the East to West.
LikeLike
J Biol Chem. 1994 Oct 28;269(43):27136-42.
Human cathepsin O. Molecular cloning from a breast carcinoma, production of the active enzyme in Escherichia coli, and expression analysis in human tissues.
Velasco G1, Ferrando AA, Puente XS, Sánchez LM, López-Otín C.
Author information
Departamento de Biología Funcional, Universidad de Oviedo, Spain.
See: https://i.imgur.com/j4J7rJS.png
LikeLike
My count so far (after following Zebedee’s lead) has the wandering Northern-blot Actin controls appearing in:
1. “Human cathepsin O. Molecular cloning from a breast carcinoma, production of the active enzyme in Escherichia coli, and expression analysis in human tissues” (1994).
J Biol Chem. 1994 Oct 28;269(43):27136-42.
“Cloning and expression analysis of a novel human serine hydrolase with sequence similarity to prokaryotic enzymes involved in the degradation of aromatic compounds” (1995)
JBC 270, 12926-12932.
10.1074/jbc.270.21.12926
“Identification and characterization of a novel human matrix metalloproteinase with unique structural characteristics, chromosomal location, and tissue distribution” (1997).
J Biol Chem. 1997 Feb 14;272(7):4281-6.
“Cathepsin Z, a novel human cysteine proteinase with a short propeptide domain and a unique chromosomal location” (1998).
J Biol Chem. 1998 Jul 3;273(27):16816-23.
“Cloning and characterization of human MMP-23, a new matrix metalloproteinase predominantly expressed in reproductive tissues and lacking conserved domains in other family members” (1999).
J Biol Chem. 1999 Feb 19;274(8):4570-6.
“Molecular cloning and structural and functional characterization of human cathepsin F, a new cysteine proteinase of the papain family with a long propeptide domain” (1999).
J Biol Chem. 1999 May 14;274(20):13800-9
“Human MT6-matrix metalloproteinase: identification, progelatinase A activation, and expression in brain tumors”.
Cancer Research (2000).
“Identification, Characterization, and Intracellular Processing of ADAM-TS12, a Novel Human Disintegrin with a Complex Structural Organization Involving Multiple Thrombospondin-1 Repeats” (2001).
Journal of Biological Chemistry (2001) : 10.1074/jbc.M100534200
“Matriptase-2, a Membrane-bound Mosaic Serine Proteinase Predominantly Expressed in Human Liver and Showing Degrading Activity against Extracellular Matrix Proteins” (2002).
Journal of Biological Chemistry (2002) – 10.1074/jbc.M203007200
“Polyserase-I, a human polyprotease with the ability to generate independent serine protease domains from a single translation product” (2003).
Proc Natl Acad Sci U S A. 2003 Aug 5; 100(16): 9185–9190.
“Human Autophagins, a Family of Cysteine Proteinases Potentially Implicated in Cell Degradation by Autophagy” (2003).
Journal of Biological Chemistry (2003) – 10.1074/jbc.M208247200
“Identification of Human Aminopeptidase O, a Novel Metalloprotease with Structural Similarity to Aminopeptidase B and Leukotriene A4 Hydrolase” (2005).
Journal of Biological Chemistry (2005) – 10.1074/jbc.M413222200
“Human Polyserase-2, a Novel Enzyme with Three Tandem Serine Protease Domains in a Single Polypeptide Chain” (2005).
Journal of Biological Chemistry (2005) – 10.1074/jbc.M409139200
“Identification and characterization of human polyserase-3, a novel protein with tandem serine-protease domains in the same polypeptide chain” (2006).
BMC Biochemistry (2006) – 10.1186/1471-2091-7-9
More precisely, there are four versions of the Actin band… one for fetal tissues with four lanes; and two for adult tissues and one for tumor cell lanes, each with eight lanes. Lanes are sometimes labelled consistently. Not all of the bands always appear in a single paper. They undergo occasional horizontal or vertical flips and stretching, and sometimes lose lanes from the ends or even the middle.
Have I missed any?
LikeLike
It would be informative to collect and put all the doubtful images of Lopez-Otin’s papers in a single place, so others could forsee the ‘impact’ of his study at a glance.
Gather images in a bundle of imgur links. Appreciate if one could check and re-put images in imgur links at pubpeer.
NF-κB activation impairs somatic cell reprogramming in ageing
Nature Cell Biology (2015) – 12 Comments
doi: 10.1038/ncb3207 issn: 1465-7392 issn: 1476-4679 pubmed: 26214134
Clara Soria-Valles , Fernando G. Osorio , Ana Gutiérrez-Fernández , Alejandro De Los Angeles , Clara Bueno , Pablo Menéndez , José I. Martín-Subero , George Q. Daley , José M. P. Freije , Carlos López-Otín
Figure 3.
Figure 6
Figure 6
Identification and Characterization of Human and Mouse Ovastacin
Journal of Biological Chemistry (2004) – 4 Comments
pubmed: 15087446 doi: 10.1074/jbc.M401588200 issn: 0021-9258 issn: 1083-351X
Víctor Quesada , Luis M. Sánchez , Jesús Alvarez , Carlos López-Otín
Figure 4.
Human Autophagins, a Family of Cysteine Proteinases Potentially Implicated in Cell Degradation by Autophagy
Journal of Biological Chemistry (2003) – 6 Comments
pubmed: 12446702 doi: 10.1074/jbc.M208247200 issn: 0021-9258 issn: 1083-351X
Guillermo Mariño , José A Uría , Xose S. Puente , Vı́ctor Quesada , Javier Bordallo , Carlos López-Otín
Figure 8
Figure 3 continued.
Human MT6-matrix metalloproteinase: identification, progelatinase A activation, and expression in brain tumors
Cancer Research (2000) – 11 Comments
pubmed: 10706098 issn: 0008-5472
G Velasco , S Cal , A Merlos-Suárez , A A Ferrando , S Alvarez , A Nakano , J Arribas , C López-Otín
Identification and characterization of human polyserase-3, a novel protein with tandem serine-protease domains in the same polypeptide chain
BMC Biochemistry (2006) – 5 Comments
pubmed: 16566820 doi: 10.1186/1471-2091-7-9 issn: 1471-2091
Santiago Cal , Juan R Peinado , María Llamazares , Víctor Quesada , Angela Moncada-Pazos , Cecilia Garabaya , Carlos López-Otín
To be continued.
LikeLike
Identification of Human Aminopeptidase O, a Novel Metalloprotease with Structural Similarity to Aminopeptidase B and Leukotriene A Hydrolase
Journal of Biological Chemistry (2005) – 2 Comments
pubmed: 15687497 doi: 10.1074/jbc.M413222200 issn: 0021-9258 issn: 1083-351X
Araceli Díaz-Perales , Víctor Quesada , Luis M. Sánchez , Alejandro P. Ugalde , María F. Suárez , Antonio Fueyo , Carlos López-Otín
LikeLike
Molecular cloning and structural and functional characterization of human cathepsin F, a new cysteine proteinase of the papain family with a long propeptide domain
Journal of Biological Chemistry (1999) – 3 Comments
pubmed: 10318784 issn: 0021-9258
I Santamaría , G Velasco , A M Pendás , A Paz , C López-Otín
Figure 5
Figure 6
bands are wandering to other papers.
Human Polyserase-2, a Novel Enzyme with Three Tandem Serine Protease Domains in a Single Polypeptide Chain
Journal of Biological Chemistry (2005) – 2 Comments
pubmed: 15536082 doi: 10.1074/jbc.M409139200 issn: 0021-9258 issn: 1083-351X
Santiago Cal , Víctor Quesada , María Llamazares , Araceli Díaz-Perales , Cecilia Garabaya , Carlos López-Otín
Figure 3
Human MT6-matrix metalloproteinase: identification, progelatinase A activation, and expression in brain tumors
Cancer Research (2000) – 12 Comments
pubmed: 10706098 issn: 0008-5472
G Velasco , S Cal , A Merlos-Suárez , A A Ferrando , S Alvarez , A Nakano , J Arribas , C López-Otí
Figure 4
NF-κB activation impairs somatic cell reprogramming in ageing
Nature Cell Biology (2015) – 13 Comments
doi: 10.1038/ncb3207 issn: 1465-7392 issn: 1476-4679 pubmed: 26214134
Clara Soria-Valles , Fernando G. Osorio , Ana Gutiérrez-Fernández , Alejandro De Los Angeles , Clara Bueno , Pablo Menéndez , José I. Martín-Subero , George Q. Daley , José M. P. Freije , Carlos López-Otín
Supplementary figure 8.
Cloning and characterization of human MMP-23, a new matrix metalloproteinase predominantly expressed in reproductive tissues and lacking conserved domains in other family members
Journal of Biological Chemistry (1999) – 1 Comment
pubmed: 9988691 issn: 0021-9258
G Velasco , A M Pendás , A Fueyo , V Knäuper , G Murphy , C López-Otín
Figure 6
Matriptase-2, a Membrane-bound Mosaic Serine Proteinase Predominantly Expressed in Human Liver and Showing Degrading Activity against Extracellular Matrix Proteins
Journal of Biological Chemistry (2002) – 7 Comments
pubmed: 12149247 doi: 10.1074/jbc.M203007200 issn: 0021-9258 issn: 1083-351X
Gloria Velasco , Santiago Cal , Victor Quesada , Luis M. Sánchez , Carlos López-Otín
Figure 8
Cathepsin Z, a novel human cysteine proteinase with a short propeptide domain and a unique chromosomal location
Journal of Biological Chemistry (1998) – 1 Comment
pubmed: 9642240 issn: 0021-9258
I Santamaría , G Velasco , A M Pendás , A Fueyo , C López-Otín
Figure 5
Human cathepsin O. Molecular cloning from a breast carcinoma, production of the active enzyme in Escherichia coli, and expression analysis in human tissues
Journal of Biological Chemistry (1994) – 1 Comment
pubmed: 7929457 issn: 0021-9258
G Velasco , A A Ferrando , X S Puente , L M Sánchez , C López-Otín
Figure 5
To be continued.
LikeLike
Not just Nature rewards data manipulation, but the European Research Council also rewards data manipulation.
Click to access ERC-2016-AdG-results-all-domains.pdf
Page 8.
ERC Advanced Grants 2016
LOPEZ OTIN Carlos
Universidad de Oviedo
University of Oviedo ES DeAge
Deconstructing Ageing: from molecular mechanisms to intervention strategies
LS4
LikeLike
Pingback: Does ERC help cheaters pay protection money? – For Better Science
J Immunol. 2012 Oct 15;189(8):4135-43. doi: 10.4049/jimmunol.1103739. Epub 2012 Sep 7.
Control of allergen-induced inflammation and hyperresponsiveness by the metalloproteinase ADAMTS-12.
Paulissen G1, El Hour M, Rocks N, Guéders MM, Bureau F, Foidart JM, Lopez-Otin C, Noel A, Cataldo DD.
Author information
Laboratory of Tumor and Developmental Biology, Interdisciplinary Group of Applied Genoproteomics-Cancer (GIGA-Cancer), University of Liège and University Hospital of Liège, 4000 Liège, Belgium.
Figure 3B. https://imgur.com/08ANZ5K
LikeLike
Am J Respir Cell Mol Biol. 2010 Sep;43(3):286-95. doi: 10.1165/rcmb.2008-0426OC. Epub 2009 Oct 20.
Matrix metalloproteinase-19 deficiency promotes tenascin-C accumulation and allergen-induced airway inflammation.
Maud M. Gueders 1,2, Stuart J. Hirst 3, Florence Quesada-Calvo 1,2, Geneviève Paulissen 1,2, Jonathan Hacha 1,2, Christine Gilles 1, Philippe Gosset 4, Renaud Louis 2, Jean-Michel Foidart 1, Carlos Lopez-Otin 5, Agnes Noël 1, and Show All…
1Laboratory of Tumor Biology and Development, and 2Department of Respiratory Diseases, GIGA-I3 and GIGA Cancer, GIGA Research, University of Liege and Centre Hospitalier Universitaire, CHU-Liege, Liege, Belgium; 3Department of Physiology, Monash University, Melbourne, Australia; 4Unité INSERM U774, Institut Pasteur de Lille, Lille, France; and 5Departamento de Bioquimica y Biologia Molecular, Instituto Universitario de Oncologia, Universidad de Oviedo, Oviedo, Spain.
Figure 6A. https://imgur.com/mLTiYJu
LikeLike
Expression of collagenase-3 in the rat ovary during the ovulatory process
Journal of Endocrinology (1996) – 1 Comment
pubmed: 8691099 issn: 0022-0795
M Balbín , A Fueyo , J M López , I Díez-Itza , G Velasco , C López-Otín
Hormonal regulation of the human pepsinogen C gene in breast cancer cells. Identification of a cis-acting element mediating its induction by androgens, glucocorticoids, and progesterone
Journal of Biological Chemistry (1996) – 1 Comment
pubmed: 8663058 issn: 0021-9258
M Balbín , C López-Otín
LikeLike
Accelerated ageing in mice deficient in Zmpste24 protease is linked to p53 signalling activation
Nature (2005) – 1 Comment
pubmed: 16079796 doi: 10.1038/nature04019 issn: 1476-4687 issn: 0028-0836
Ignacio Varela , Juan Cadiñanos , Alberto M Pendás , Ana Gutiérrez-Fernández , Alicia R Folgueras , Luis M Sánchez , Zhongjun Zhou , Francisco J Rodríguez , Colin L Stewart , José A Vega , Karl Tryggvason , José M P Freije , Carlos López-Otín
Control of Allergen-Induced Inflammation and Hyperresponsiveness by the Metalloproteinase ADAMTS-12
The Journal of Immunology (2012) – 2 Comments
pubmed: 22962682 doi: 10.4049/jimmunol.1103739 issn: 1550-6606 issn: 0022-1767
Geneviève Paulissen , Mehdi El Hour , Natacha Rocks , Maud M Guéders , Fabrice Bureau , Jean-Michel Foidart , Carlos Lopez-Otin , Agnès Noel , Didier D Cataldo
Figure 3
Matrix Metalloproteinase-19 Deficiency Promotes Tenascin-C Accumulation and Allergen-Induced Airway Inflammation
American Journal of Respiratory Cell and Molecular Biology (2010) – 2 Comments
pubmed: 19843707 doi: 10.1165/rcmb.2008-0426OC issn: 1535-4989 issn: 1044-1549
Maud M. Gueders , Stuart J. Hirst , Florence Quesada-Calvo , Geneviève Paulissen , Jonathan Hacha , Christine Gilles , Philippe Gosset , Renaud Louis , Jean-Michel Foidart , Carlos Lopez-Otin , Agnes Noël , Didier D. Cataldo
Figure 6
Identification and characterization of human MT5-MMP, a new membrane-bound activator of progelatinase a overexpressed in brain tumors
Cancer Research (1999) – 5 Comments
pubmed: 10363975 issn: 0008-5472
E Llano , A M Pendás , J P Freije , A Nakano , V Knäuper , G Murphy , C López-Otin
Figure 5
LikeLike
Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases
Biochemical and Biophysical Research Communications – 1 Comment
doi: 10.1016/j.bbrc.2003.12.050 issn: 0006-291X
Vı́ctor Quesada , Araceli Dı́az-Perales , Ana Gutiérrez-Fernández , Cecilia Garabaya , Santiago Cal , Carlos López-Otı́n
LikeLike
Nature. 2005 Sep 22;437(7058):564-8. Epub 2005 Aug 3.
Accelerated ageing in mice deficient in Zmpste24 protease is linked to p53 signalling activation.
Varela I1, Cadiñanos J, Pendás AM, Gutiérrez-Fernández A, Folgueras AR, Sánchez LM, Zhou Z, Rodríguez FJ, Stewart CL, Vega JA, Tryggvason K, Freije JM, López-Otín C.
Author information
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncología, Universidad de Oviedo, 33006 Oviedo, Spain.
Much more similar than you would expect.
See: https://i.imgur.com/Ttj0XoS.png
LikeLike
According to “Fundación GADEA por la Ciencia”, http://gadeaciencia.org/en/the-foundation/board/
of which the President Scientific Advisory Counsil (website’s typo), is the prominent scientist Mariano Barbacid, and the scientific advisory council http://gadeaciencia.org/en/scientific-advisory-council/includes of which includes such prominent members as:-
Out of Spain section.
1. Carlos Cordon-Cardo, (2017 retraction of 2003 paper http://mcb.asm.org/content/37/18/e00365-17.short “Digital experts have informed us that some of the published data in this paper were very likely digitally manipulated.”, but could not figure it out himself, which does suggest the question of his being about to advise anybody else about authenticity, or being able to judge data),
2. Salvador “4 retractions” Moncada, who had 3 retractions in his group at UCL, https://retractionwatch.com/2012/09/04/three-retractions-for-university-college-london-mitochondrial-biologist-for-inappropriate-figure-manipulations/, under somebody’s nose does not suggest competency at being a judge, and one retraction delayed 17 years at Harvard. https://retractionwatch.com/2012/09/10/ori-findings-lead-to-two-retractions-nearly-17-years-later/, this particular retraction: http://www.jimmunol.org/content/189/6/3260.full
In Spain section.
3. Manel Esteller, who is well documented at Pubpeer:
https://pubpeer.com/search?q=manel+estellerpubpeer
3. Pura Muñoz-Canoves, who is also well-documented at Pubpeer:
https://pubpeer.com/search?q=Mu%C3%B1oz-Canoves
Last, but not least. Carlos Lopez-Otin.
Carlos Lopez-Otin
http://gadeaciencia.org/teams/lopez-otin-carlos-2/
“The work of López-Otín’s laboratory has yielded the identification of more than 60 novel human proteases dysregulated in cancer and defined their functional role in cancer and in other pathological processes, including the devastating syndromes of accelerated aging.”
His data are in conflict with those claims.
It is not obvious if being a “Team” means that you are funded by “Fundación GADEA por la Ciencia”.
LikeLike
Cancer Res. 1996 Apr 15;56(8):1746-50.
Cloning and expression analysis of human bleomycin hydrolase, a cysteine proteinase involved in chemotherapy resistance.
Ferrando AA1, Velasco G, Campo E, Lopez-Otin C.
Author information
Departmento de Bioquimica y Biologia Molecular, Facultad de Medicina, Universidad de Oviedo, Spain.
See: https://i.imgur.com/kBKnOAQ.png
LikeLike
Blood. 1997 Jan 1;89(1):272-80.
Deletions and loss of expression of p16INK4a and p21Waf1 genes are associated with aggressive variants of mantle cell lymphomas.
Pinyol M1, Hernandez L, Cazorla M, Balbín M, Jares P, Fernandez PL, Montserrat E, Cardesa A, Lopez-Otín C, Campo E.
Author information
Department of Anatomic Pathology, Hospital Clinic Provincial, University of Barcelona, Spain.
Figure 1. Differential splicing between panels dilutes meaning.
See: https://i.imgur.com/znY6avC.png
LikeLike
Pingback: The Perennial Northern Blot of Lopez-Otin – For Better Science
http://www.elmundo.es/ciencia/2016/07/24/579272dc268e3e8a788b4654.html
“Carlos López Otín: “España es una sociedad sin afición por la ciencia” “.
He should speak for himself.
Is it a plea for help?
LikeLike
J Biol Chem. 2012 Nov 16;287(47):39554-63. doi: 10.1074/jbc.M112.408625. Epub 2012 Sep 27.
ADAMTS-12 metalloprotease is necessary for normal inflammatory response.
Moncada-Pazos A1, Obaya AJ, Llamazares M, Heljasvaara R, Suárez MF, Colado E, Noël A, Cal S, López-Otín C.
Author information
Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Oncología (IUOPA), Universidad de Oviedo, 33006 Oviedo, Spain.
2018 correction.
http://www.jbc.org/content/293/29/11648.short
Fig. 3C did not indicate the borders between different sections of the same gel for the calprotectin, S100A8, and S100A9 immunoblots from day 8. The actin immunoblots were electrophoresed on parallel gels. This error has now been corrected and does not affect the results or conclusions of this work.
LikeLike
J Biol Chem. 2008 Apr 4;283(14):9465-74. doi: 10.1074/jbc.M709522200. Epub 2008 Feb 1.
Collagenase-2 deficiency or inhibition impairs experimental autoimmune encephalomyelitis in mice.
Folgueras AR1, Fueyo A, García-Suárez O, Cox J, Astudillo A, Tortorella P, Campestre C, Gutiérrez-Fernández A, Fanjul-Fernández M, Pennington CJ, Edwards DR, Overall CM, López-Otín C.
Author information
1
Departamento de Bioquímica y Biología Molecular, and Biología Funcional, Facultad de Medicina, Instituto Universitario de Oncología, Universidad de Oviedo, and Servicio de Anatomía Patológica, Hospital Central de Asturias, Oviedo 33006, Spain.
2018 correction.
http://www.jbc.org/content/293/30/11968.short
For clarity, upon assembly of Figs. 4, 5A, and 8B, corresponding to a dissection of mouse spinal cords, torn fragments of adjacent tissue that were incompletely removed during manual dissection were eliminated digitally, leaving only the spinal cord tissue under study. The unprocessed versions of these figures are shown without this modification. Additionally, an incorrect image was shown in Fig. 4O. These errors have now been corrected and do not affect the results or conclusions of this work.
LikeLike
The issues need MORE attension. Integrity of JBC could be in doubt. Unfortunate that Carlos Lopez-Otin escapes from his misconduct dealt doen with mere correction.
@jbiolchem
LikeLike