In Spain, there seems to be a tradition of reacting to emerging evidence of data manipulation with handing out prestigious awards to authors of these papers. This is how we were all taught that all the PubPeer evidence matters nothing whatsoever, as Pura Munoz-Canoves, Maria Pia Cosma and Manel Esteller were celebrated with prizes and grants for their research achievements (read here , here and here).  Now there is a new popular academic hero in Spain, Carlos López-Otín, Professor of Biochemistry and Molecular Biology at the University of Oviedo, and EMBO member. Lopez-Otin was now awarded a 2017 Mentoring Award,  presented to him by Sir Philip Campbell, on behalf of his journal Nature, which Editor-in-Chief Sir Philip has been for the last 22 years.

The journal Nature remains the highest authority in science, this Mentoring Award is Sir Philip’s parting present to the Nature-reading scientific community, as he is being promoted inside Springer Nature’s publishing hierarchy. It is in this way a statement of what Nature and its publisher think of research integrity. Lopez-Otin’s evidence record on PubPeer is telling enough, however most of the evidence of digitally manipulated gel images there was posted around 2-3 months ago, by the pseudonymous Claire Francis. Maybe the evidence appeared too late, and the award decision couldn’t be stopped anymore. Which would explain why Nature deleted their own tweet announcing the mentorship awards after I replied to it with PubPeer evidence on Lopez-Otin’s papers. According to another Nature website, which was for some reason also deleted, the closing date for nominations was Monday 31 July 2017. So I share some examples below, all are from papers where Lopez-Otin is last and corresponding author.

image-1506331676940
PubPeer evidence for Velasco et al Cancer Research 2000 and Velasco et al JBC 2002. It is obvious controls were run on separate gels, the-reused, and inappropriately inverted. Boxes highlight suspiciously similar areas of the background.

Nature tweeted the article about mentorship awards in Spain, to which I replied. Unlike with other social media, you cannot remove replies to your tweets if you disagree with those. Pop, the original Nature tweet was deleted, Nature followers on Twitter were then spared reading this filth:

The Nature site which invited award nomination from Spain, was gone as well. This was the awarding committee, according to residues that site left Google search and this source:

Chair of the judges: Josep Penuelas, Center for Ecological Research and Forestry Applications (CREAF) – National Research Council (CSIC), Barcelona

Judging panel:
Alison Abbott, Nature, Munich, Germany
Emilia R. Solano, CIEMAT, Madrid, Spain
Juan Lerma, Instituto de Neurociencias de Alicante – UMH, Alicante, Spain
Mariano Barbacid, Centro Nacional de Investigaciones Oncológicas, Madrid, Spain
Pilar Ruiz Lapuente, Institute of Cosmos Sciences, University of Barcelona, Barcelona, Spain

image-1506685431120
PubPeer evidence for suspected gel band duplication (highlighted with colour boxes)  in Mariño et al, JBC 2007

For some reason, jury member Alison Abbot failed to mention her decisive role in her follow-up Nature article, while the past one listing her as member of the judging panel was deleted.

screenshot-www.google.de-2017-12-19-12-23-40
All which is left of the deleted Nature site, a Google search blurb

When issuing the award, Sir Philip said about Lopez-Otin:

Spain’s great examples are no exception – they are extraordinary in their ability to nurture emerging scientists of great diversity.”

Extraordinary re-use of a loading control indeed, in this paper from Lopez-Otin lab:

image-1506619193651
PubPeer evidence for Jiménez et al, JCB 2001. Not only does the loading control appear to be re-used for different experiments, the first band on that gel is clearly different,

Alison Abbot, who was one of the judges, later wrote in this Nature article:

“López-Otín’s mentees praised his ability to draw out and encourage even the shyest lab member. “Carlos gave me the strength to pursue an independent career as a principal investigator,” one wrote. “He made me feel that my career was a priority for him, and it is thanks to him that my career has advanced further than I would ever have imagined.” Another noted how López-Otín had encouraged trainees to read widely and think big. “Carlos told me that ground-breaking work comes from broad scientific knowledge, while overspecialization is the path to small science.”

Nominators said that López-Otín insisted on celebrating every accepted paper or project in the lab and was widely generous with advice”.

One wonders, how was this copy-pasting on the background, apparently required in order to get this paper accepted, celebrated?

image-1506419828955
PubPeer evidence for Llano et al, JBC 2008. This figure is most obviously crudely manipulated, with duplicated parts of background. The image is not even contrast-enhanced, only boxes and arrows added to highlight manipulations.

Another quote from the Nature article by Alison Abbot:

“Carlos has been a constant catalyst of scientific activities in Asturias, a region marked by decades of economic and social distress,” wrote one of his mentees”.

In this vein, was prompting people to clone gel bands part of those catalytic activities of the mentorship award-winner Lopez-Otin?

image-1506683267923
PubPeer evidence for Díaz-Perales et al JBC 2005. heavy lane splicing aside, one lane in GAPD seems to feature three times on the same gel.

Even Sir Philip’s own Nature family wasn’t spared of Lopez-Otis irregularities. This PubPeer post discusses Soria-Valles et al, Nature Cell Biology, 2015, and how the provided original blot images in the supplementary data do not match their published figure counterparts.

 


 

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48 thoughts on “Nature rewards data manipulation with a Mentoring Award

  1. For sure artistic creativity must be compensated….indeed a good part of a PhD in Spain is dedicated how to do the best Adobe Photoshop artistic jobs….

    Like

    1. Well Ana,

      Sounds like you speak from experience, as, to the best of my knowldege, you are supporting such an UNTRUE statement by no evidence appart from your own PhD (and perhaps someone else’s in your environment?) in Spain.
      Bottom line: you are just fabricating the data on which you based your conclusions. Therefore, in the light of your affirmation, I could probably say that you did a great PhD in Spain, right?. Good for you then!!

      Like

      1. Dear Manuel
        Indeed I did a great, fair and clean PhD in Spain and I am truly thankful to a couple of Spanish who helped me in their painful crusade against their own system
        Everybody knows about how the system works in Spain and most of the academics are afraid of talking fearing represalies
        In Portugal the system is similar, fortunately we have an research fellows association who intends to defend their rights, ABIC with many difficulties

        Like

      2. Dear Ana,
        I cannot do other thing that support Manuel and feel sorry for the pathetic environment you had on your PhD, mine, also in Spain, was full of amazing scientists, that were able to publish in very good journal with much less support than other countries. I learnt very good science, how to be a good scientist, and testimony of that is the good job I am doing outside Spain. I’m super proud, and it was here in UK the first time I faced lies in science and had to live with it. You are an arrogant and an ignorant by thinking that you were the only honest scientist in Spain or one of the “few”…keep doing that thinking you are the best, that kind of humility and respect to others is exactly what a good scientist should be…

        Like

  2. You have to congratulate Nature on bringing the data manipulation to public attention.
    Nature may be unwitting, but it does have high impact.

    Like

      1. Dear Isabel,

        I am still waiting you to name 20 “amazing scientists” in Spain.
        It should not be difficult as you wrote that “Spain, was full of amazing scientists”.
        If you cannot name 20 where have they gone?

        Like

      2. I hope we could end this debate by agreeing there are in fact quite a lot of very good scientists in Spain, but under current conditions they must live in fear of their dishonest peers who accumulate power, grants and awards. An award given to these cheaters is not just a meaningless cup or a medal. It is a sign who enjoys protection from the very top. Spain as a former oppressive dictatorship surely knows how this works.

        Like

  3. Antonio Garcia de Herreros.
    https://www.imim.cat/programesrecerca/cancer/en_ubcm.html

    https://pubpeer.com/publications/BDEB1BEC0F27AC6D5AFDD0D0E980F7
    https://pubpeer.com/publications/D9AB005FB5B07483BC12C2C0DFB454
    https://pubpeer.com/publications/5A785B0CF23403E24C25D3C43111FB
    https://pubpeer.com/publications/981BDA5DB6DFD80DCC41AEF1808BE9
    https://pubpeer.com/publications/BEB69B48326037191631A1B13D607C
    https://pubpeer.com/publications/4166CB88F57104A834DDEF71515EEC
    https://pubpeer.com/publications/A8F3E69DACCF638411E66257F47A9F
    https://pubpeer.com/publications/447EC5BFD11D3D1D947100DAB271F9
    https://pubpeer.com/publications/2EB431B880F997FBCDB5A4E48927FD
    https://pubpeer.com/publications/034CC3FA793059AD615B5A5D80BD84
    https://pubpeer.com/publications/95D7EDC45C43706AB46E45AE03F1A1
    https://pubpeer.com/publications/7A2D9BBDCE85623CB4824CA04077E0
    https://pubpeer.com/publications/07643B5654697153BE6504D2C3F966
    https://pubpeer.com/publications/2F0AF28466D2E1D31510ACC72794F1
    https://pubpeer.com/publications/8A9B324060E1673639C5114CC0F3C5
    https://pubpeer.com/publications/77ACF5E0CB3F2BB5DEAF2019B0CCE3
    https://pubpeer.com/publications/C82AD606E1FE4DE152DA1D1796DE7B
    https://pubpeer.com/publications/AE72300768ECE0EA987B4842EB30CA
    https://pubpeer.com/publications/0E155718533A0156BBEAD8E8D59A0C
    https://pubpeer.com/publications/0A32B29DEA2D2B0A10F4F362F55CD4
    https://pubpeer.com/publications/E2D2996937DCDDAF9559725FDFDC95
    https://pubpeer.com/publications/0E99B1B3B43B7D6917347AAA23EE7B
    https://pubpeer.com/publications/42E81CC060E1BEA09DB2F7AAC9E484
    https://pubpeer.com/publications/A62C2A767B8A9A60229DD116BCBB0D
    https://pubpeer.com/publications/03C59A9C6383C9E70C9EEA7E056336
    https://pubpeer.com/publications/DD8A003880FB4B4E2CE5218FD971D8
    https://pubpeer.com/publications/7B8A5F590BF1468283CC3FE7D6BBCA
    https://pubpeer.com/publications/E3471375942FBF3B5CBA8C3AC0C551
    https://pubpeer.com/publications/7D64B99471B2AE86604500FC8D6569

    Like

  4. J Biol Chem. 2004 Jun 18;279(25):26627-34. Epub 2004 Apr 15.
    Identification and characterization of human and mouse ovastacin: a novel metalloproteinase similar to hatching enzymes from arthropods, birds, amphibians, and fish.
    Quesada V1, Sánchez LM, Alvarez J, López-Otín C.
    Author information

    1
    Departamento de Bioquimica y Biologia Molecular and Morfologia y Biologia Celular, Facultad de Medicina, Instituto Universitario de Oncologia, Universidad de Oviedo, 33006-Oviedo, Spain.

    Figure 4. https://imgur.com/ZzWbrk5

    Figure 3, see: https://pubpeer.com/publications/A2392B5A5FD19456E8AD74A68FCF80

    Like

  5. NF-κB activation impairs somatic cell reprogramming in ageing
    Nature Cell Biology (2015) – 4 Comments
    doi: 10.1038/ncb3207 issn: 1465-7392 issn: 1476-4679 pubmed: 26214134

    Clara Soria-Valles , Fernando G. Osorio , Ana Gutiérrez-Fernández , Alejandro De Los Angeles , Clara Bueno , Pablo Menéndez , José I. Martín-Subero , George Q. Daley , José M. P. Freije , Carlos López-Otín

    https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18

    Figure 2, 6, 7.
    https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18#1

    Figure 3.

    https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18#3

    Figure 7.
    https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18#2

    Like

  6. Still arguments continue on the paper of Soria-Valles et al…
    NF-κB activation impairs somatic cell reprogramming in ageing
    Nature Cell Biology (2015) – 4 Comments
    doi: 10.1038/ncb3207 issn: 1465-7392 issn: 1476-4679 pubmed: 26214134
    Clara Soria-Valles , Fernando G. Osorio , Ana Gutiérrez-Fernández , Alejandro De Los Angeles , Clara Bueno , Pablo Menéndez , José I. Martín-Subero , George Q. Daley , José M. P. Freije , Carlos López-Otín

    https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18#10

    Figure 6

    Supplementary figure 6
    https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18#9

    Supplementary figure 8
    https://pubpeer.com/publications/836AB3A8AB4FD562A2D4CBFBF8ED18#5

    Like

  7. Similarities between three Lopez-Otin papers:-

    Cancer Res. 2000 Feb 15;60(4):877-82.
    Human MT6-matrix metalloproteinase: identification, progelatinase A activation, and expression in brain tumors.
    Velasco G1, Cal S, Merlos-Suárez A, Ferrando AA, Alvarez S, Nakano A, Arribas J, López-Otín C.
    Author information
    Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina, Universidad de Oviedo, Spain.
    J Biol Chem. 2002 Oct 4;277(40):37637-46. Epub 2002 Jul 30.
    Matriptase-2, a membrane-bound mosaic serine proteinase predominantly expressed in human liver and showing degrading activity against extracellular matrix proteins.
    Velasco G1, Cal S, Quesada V, Sánchez LM, López-Otín C.
    Author information
    Departamento de Bioquimica y Biologia Molecular, Instituto Universitario de Oncologia, Universidad de Oviedo, 33006 Oviedo, Spain.
    J Biol Chem. 2003 Feb 7;278(6):3671-8. Epub 2002 Nov 21.
    Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy.
    Mariño G1, Uría JA, Puente XS, Quesada V, Bordallo J, López-Otín C.
    Author information
    Departamento de Bioquimíca y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncología, Universidad de Oviedo, Spain.

    See: https://i.imgur.com/PEdywou.png

    Like

  8. J Biol Chem. 2005 Jan 21;280(3):1953-61. Epub 2004 Nov 9.
    Human polyserase-2, a novel enzyme with three tandem serine protease domains in a single polypeptide chain.
    Cal S1, Quesada V, Llamazares M, Díaz-Perales A, Garabaya C, López-Otín C.
    Author information
    1
    Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncología, Universidad de Oviedo, 33006 Oviedo, Spain.

    Sample order differences between other papers and this one outlined in orange.
    There are examples of re-use with sample order same.

    See: https://imgur.com/D25yKrt

    Like

  9. J Biol Chem. 1999 Feb 19;274(8):4570-6.
    Cloning and characterization of human MMP-23, a new matrix metalloproteinase predominantly expressed in reproductive tissues and lacking conserved domains in other family members.
    Velasco G1, Pendás AM, Fueyo A, Knäuper V, Murphy G, López-Otín C.
    Author information
    Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain.

    See: https://i.imgur.com/iQugL8A.png

    Like

    1. Treaty of Tordesillas divided the newly “discovered” lands outside Europe between the Portuguese Empire and the Crown of Castile (present day Spain), along a meridian 370 leagues west of the Cape Verde islands, off the west coast of Africa. In effect, Portugal colonized what is now Brazil, Spain colonized most of the rest of South America. In this case we are seeing Spain. The spreading goes from the East to West.

      Like

  10. J Biol Chem. 1994 Oct 28;269(43):27136-42.
    Human cathepsin O. Molecular cloning from a breast carcinoma, production of the active enzyme in Escherichia coli, and expression analysis in human tissues.
    Velasco G1, Ferrando AA, Puente XS, Sánchez LM, López-Otín C.
    Author information
    Departamento de Biología Funcional, Universidad de Oviedo, Spain.

    See: https://i.imgur.com/j4J7rJS.png

    Like

  11. My count so far (after following Zebedee’s lead) has the wandering Northern-blot Actin controls appearing in:
    1. “Human cathepsin O. Molecular cloning from a breast carcinoma, production of the active enzyme in Escherichia coli, and expression analysis in human tissues” (1994).
    J Biol Chem. 1994 Oct 28;269(43):27136-42.

    “Cloning and expression analysis of a novel human serine hydrolase with sequence similarity to prokaryotic enzymes involved in the degradation of aromatic compounds” (1995)
    JBC 270, 12926-12932.
    10.1074/jbc.270.21.12926
    “Identification and characterization of a novel human matrix metalloproteinase with unique structural characteristics, chromosomal location, and tissue distribution” (1997).
    J Biol Chem. 1997 Feb 14;272(7):4281-6.
    “Cathepsin Z, a novel human cysteine proteinase with a short propeptide domain and a unique chromosomal location” (1998).
    J Biol Chem. 1998 Jul 3;273(27):16816-23.
    “Cloning and characterization of human MMP-23, a new matrix metalloproteinase predominantly expressed in reproductive tissues and lacking conserved domains in other family members” (1999).
    J Biol Chem. 1999 Feb 19;274(8):4570-6.
    “Molecular cloning and structural and functional characterization of human cathepsin F, a new cysteine proteinase of the papain family with a long propeptide domain” (1999).
    J Biol Chem. 1999 May 14;274(20):13800-9
    “Human MT6-matrix metalloproteinase: identification, progelatinase A activation, and expression in brain tumors”.
    Cancer Research (2000).
    “Identification, Characterization, and Intracellular Processing of ADAM-TS12, a Novel Human Disintegrin with a Complex Structural Organization Involving Multiple Thrombospondin-1 Repeats” (2001).
    Journal of Biological Chemistry (2001) : 10.1074/jbc.M100534200
    “Matriptase-2, a Membrane-bound Mosaic Serine Proteinase Predominantly Expressed in Human Liver and Showing Degrading Activity against Extracellular Matrix Proteins” (2002).
    Journal of Biological Chemistry (2002) – 10.1074/jbc.M203007200
    “Polyserase-I, a human polyprotease with the ability to generate independent serine protease domains from a single translation product” (2003).
    Proc Natl Acad Sci U S A. 2003 Aug 5; 100(16): 9185–9190.
    “Human Autophagins, a Family of Cysteine Proteinases Potentially Implicated in Cell Degradation by Autophagy” (2003).
    Journal of Biological Chemistry (2003) – 10.1074/jbc.M208247200
    “Identification of Human Aminopeptidase O, a Novel Metalloprotease with Structural Similarity to Aminopeptidase B and Leukotriene A4 Hydrolase” (2005).
    Journal of Biological Chemistry (2005) – 10.1074/jbc.M413222200
    “Human Polyserase-2, a Novel Enzyme with Three Tandem Serine Protease Domains in a Single Polypeptide Chain” (2005).
    Journal of Biological Chemistry (2005) – 10.1074/jbc.M409139200
    “Identification and characterization of human polyserase-3, a novel protein with tandem serine-protease domains in the same polypeptide chain” (2006).

    BMC Biochemistry (2006) – 10.1186/1471-2091-7-9

    More precisely, there are four versions of the Actin band… one for fetal tissues with four lanes; and two for adult tissues and one for tumor cell lanes, each with eight lanes. Lanes are sometimes labelled consistently. Not all of the bands always appear in a single paper. They undergo occasional horizontal or vertical flips and stretching, and sometimes lose lanes from the ends or even the middle.

    Have I missed any?

    Like

  12. It would be informative to collect and put all the doubtful images of Lopez-Otin’s papers in a single place, so others could forsee the ‘impact’ of his study at a glance.
    Gather images in a bundle of imgur links. Appreciate if one could check and re-put images in imgur links at pubpeer.

    NF-κB activation impairs somatic cell reprogramming in ageing
    Nature Cell Biology (2015) – 12 Comments
    doi: 10.1038/ncb3207 issn: 1465-7392 issn: 1476-4679 pubmed: 26214134

    Clara Soria-Valles , Fernando G. Osorio , Ana Gutiérrez-Fernández , Alejandro De Los Angeles , Clara Bueno , Pablo Menéndez , José I. Martín-Subero , George Q. Daley , José M. P. Freije , Carlos López-Otín

    Figure 3.

    Figure 6

    Figure 6

    Identification and Characterization of Human and Mouse Ovastacin
    Journal of Biological Chemistry (2004) – 4 Comments
    pubmed: 15087446 doi: 10.1074/jbc.M401588200 issn: 0021-9258 issn: 1083-351X

    Víctor Quesada , Luis M. Sánchez , Jesús Alvarez , Carlos López-Otín

    Figure 4.

    Human Autophagins, a Family of Cysteine Proteinases Potentially Implicated in Cell Degradation by Autophagy
    Journal of Biological Chemistry (2003) – 6 Comments
    pubmed: 12446702 doi: 10.1074/jbc.M208247200 issn: 0021-9258 issn: 1083-351X

    Guillermo Mariño , José A Uría , Xose S. Puente , Vı́ctor Quesada , Javier Bordallo , Carlos López-Otín

    Figure 8

    Figure 3 continued.

    Human MT6-matrix metalloproteinase: identification, progelatinase A activation, and expression in brain tumors
    Cancer Research (2000) – 11 Comments
    pubmed: 10706098 issn: 0008-5472

    G Velasco , S Cal , A Merlos-Suárez , A A Ferrando , S Alvarez , A Nakano , J Arribas , C López-Otín

    Identification and characterization of human polyserase-3, a novel protein with tandem serine-protease domains in the same polypeptide chain
    BMC Biochemistry (2006) – 5 Comments
    pubmed: 16566820 doi: 10.1186/1471-2091-7-9 issn: 1471-2091

    Santiago Cal , Juan R Peinado , María Llamazares , Víctor Quesada , Angela Moncada-Pazos , Cecilia Garabaya , Carlos López-Otín

    To be continued.

    Like

    1. Identification of Human Aminopeptidase O, a Novel Metalloprotease with Structural Similarity to Aminopeptidase B and Leukotriene A Hydrolase
      Journal of Biological Chemistry (2005) – 2 Comments
      pubmed: 15687497 doi: 10.1074/jbc.M413222200 issn: 0021-9258 issn: 1083-351X

      Araceli Díaz-Perales , Víctor Quesada , Luis M. Sánchez , Alejandro P. Ugalde , María F. Suárez , Antonio Fueyo , Carlos López-Otín

      Like

      1. Molecular cloning and structural and functional characterization of human cathepsin F, a new cysteine proteinase of the papain family with a long propeptide domain
        Journal of Biological Chemistry (1999) – 3 Comments
        pubmed: 10318784 issn: 0021-9258

        I Santamaría , G Velasco , A M Pendás , A Paz , C López-Otín

        Figure 5

        Figure 6
        bands are wandering to other papers.

        Human Polyserase-2, a Novel Enzyme with Three Tandem Serine Protease Domains in a Single Polypeptide Chain
        Journal of Biological Chemistry (2005) – 2 Comments
        pubmed: 15536082 doi: 10.1074/jbc.M409139200 issn: 0021-9258 issn: 1083-351X

        Santiago Cal , Víctor Quesada , María Llamazares , Araceli Díaz-Perales , Cecilia Garabaya , Carlos López-Otín

        Figure 3

        Human MT6-matrix metalloproteinase: identification, progelatinase A activation, and expression in brain tumors
        Cancer Research (2000) – 12 Comments
        pubmed: 10706098 issn: 0008-5472

        G Velasco , S Cal , A Merlos-Suárez , A A Ferrando , S Alvarez , A Nakano , J Arribas , C López-Otí

        Figure 4

        NF-κB activation impairs somatic cell reprogramming in ageing
        Nature Cell Biology (2015) – 13 Comments
        doi: 10.1038/ncb3207 issn: 1465-7392 issn: 1476-4679 pubmed: 26214134

        Clara Soria-Valles , Fernando G. Osorio , Ana Gutiérrez-Fernández , Alejandro De Los Angeles , Clara Bueno , Pablo Menéndez , José I. Martín-Subero , George Q. Daley , José M. P. Freije , Carlos López-Otín

        Supplementary figure 8.

        Cloning and characterization of human MMP-23, a new matrix metalloproteinase predominantly expressed in reproductive tissues and lacking conserved domains in other family members
        Journal of Biological Chemistry (1999) – 1 Comment
        pubmed: 9988691 issn: 0021-9258

        G Velasco , A M Pendás , A Fueyo , V Knäuper , G Murphy , C López-Otín

        Figure 6

        Matriptase-2, a Membrane-bound Mosaic Serine Proteinase Predominantly Expressed in Human Liver and Showing Degrading Activity against Extracellular Matrix Proteins
        Journal of Biological Chemistry (2002) – 7 Comments
        pubmed: 12149247 doi: 10.1074/jbc.M203007200 issn: 0021-9258 issn: 1083-351X

        Gloria Velasco , Santiago Cal , Victor Quesada , Luis M. Sánchez , Carlos López-Otín

        Figure 8

        Cathepsin Z, a novel human cysteine proteinase with a short propeptide domain and a unique chromosomal location
        Journal of Biological Chemistry (1998) – 1 Comment
        pubmed: 9642240 issn: 0021-9258

        I Santamaría , G Velasco , A M Pendás , A Fueyo , C López-Otín

        Figure 5

        Human cathepsin O. Molecular cloning from a breast carcinoma, production of the active enzyme in Escherichia coli, and expression analysis in human tissues
        Journal of Biological Chemistry (1994) – 1 Comment
        pubmed: 7929457 issn: 0021-9258

        G Velasco , A A Ferrando , X S Puente , L M Sánchez , C López-Otín

        Figure 5

        To be continued.

        Like

  13. J Immunol. 2012 Oct 15;189(8):4135-43. doi: 10.4049/jimmunol.1103739. Epub 2012 Sep 7.
    Control of allergen-induced inflammation and hyperresponsiveness by the metalloproteinase ADAMTS-12.
    Paulissen G1, El Hour M, Rocks N, Guéders MM, Bureau F, Foidart JM, Lopez-Otin C, Noel A, Cataldo DD.
    Author information
    Laboratory of Tumor and Developmental Biology, Interdisciplinary Group of Applied Genoproteomics-Cancer (GIGA-Cancer), University of Liège and University Hospital of Liège, 4000 Liège, Belgium.

    Figure 3B. https://imgur.com/08ANZ5K

    Like

  14. Am J Respir Cell Mol Biol. 2010 Sep;43(3):286-95. doi: 10.1165/rcmb.2008-0426OC. Epub 2009 Oct 20.
    Matrix metalloproteinase-19 deficiency promotes tenascin-C accumulation and allergen-induced airway inflammation.
    Maud M. Gueders 1,2, Stuart J. Hirst 3, Florence Quesada-Calvo 1,2, Geneviève Paulissen 1,2, Jonathan Hacha 1,2, Christine Gilles 1, Philippe Gosset 4, Renaud Louis 2, Jean-Michel Foidart 1, Carlos Lopez-Otin 5, Agnes Noël 1, and Show All…
    1Laboratory of Tumor Biology and Development, and 2Department of Respiratory Diseases, GIGA-I3 and GIGA Cancer, GIGA Research, University of Liege and Centre Hospitalier Universitaire, CHU-Liege, Liege, Belgium; 3Department of Physiology, Monash University, Melbourne, Australia; 4Unité INSERM U774, Institut Pasteur de Lille, Lille, France; and 5Departamento de Bioquimica y Biologia Molecular, Instituto Universitario de Oncologia, Universidad de Oviedo, Oviedo, Spain.

    Figure 6A. https://imgur.com/mLTiYJu

    Like

  15. Expression of collagenase-3 in the rat ovary during the ovulatory process
    Journal of Endocrinology (1996) – 1 Comment
    pubmed: 8691099 issn: 0022-0795

    M Balbín , A Fueyo , J M López , I Díez-Itza , G Velasco , C López-Otín

    Hormonal regulation of the human pepsinogen C gene in breast cancer cells. Identification of a cis-acting element mediating its induction by androgens, glucocorticoids, and progesterone
    Journal of Biological Chemistry (1996) – 1 Comment
    pubmed: 8663058 issn: 0021-9258

    M Balbín , C López-Otín

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    1. Accelerated ageing in mice deficient in Zmpste24 protease is linked to p53 signalling activation
      Nature (2005) – 1 Comment
      pubmed: 16079796 doi: 10.1038/nature04019 issn: 1476-4687 issn: 0028-0836

      Ignacio Varela , Juan Cadiñanos , Alberto M Pendás , Ana Gutiérrez-Fernández , Alicia R Folgueras , Luis M Sánchez , Zhongjun Zhou , Francisco J Rodríguez , Colin L Stewart , José A Vega , Karl Tryggvason , José M P Freije , Carlos López-Otín

      Control of Allergen-Induced Inflammation and Hyperresponsiveness by the Metalloproteinase ADAMTS-12
      The Journal of Immunology (2012) – 2 Comments
      pubmed: 22962682 doi: 10.4049/jimmunol.1103739 issn: 1550-6606 issn: 0022-1767

      Geneviève Paulissen , Mehdi El Hour , Natacha Rocks , Maud M Guéders , Fabrice Bureau , Jean-Michel Foidart , Carlos Lopez-Otin , Agnès Noel , Didier D Cataldo

      Figure 3

      Matrix Metalloproteinase-19 Deficiency Promotes Tenascin-C Accumulation and Allergen-Induced Airway Inflammation
      American Journal of Respiratory Cell and Molecular Biology (2010) – 2 Comments
      pubmed: 19843707 doi: 10.1165/rcmb.2008-0426OC issn: 1535-4989 issn: 1044-1549

      Maud M. Gueders , Stuart J. Hirst , Florence Quesada-Calvo , Geneviève Paulissen , Jonathan Hacha , Christine Gilles , Philippe Gosset , Renaud Louis , Jean-Michel Foidart , Carlos Lopez-Otin , Agnes Noël , Didier D. Cataldo

      Figure 6

      Identification and characterization of human MT5-MMP, a new membrane-bound activator of progelatinase a overexpressed in brain tumors
      Cancer Research (1999) – 5 Comments
      pubmed: 10363975 issn: 0008-5472

      E Llano , A M Pendás , J P Freije , A Nakano , V Knäuper , G Murphy , C López-Otin

      Figure 5

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      1. Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases
        Biochemical and Biophysical Research Communications – 1 Comment
        doi: 10.1016/j.bbrc.2003.12.050 issn: 0006-291X

        Vı́ctor Quesada , Araceli Dı́az-Perales , Ana Gutiérrez-Fernández , Cecilia Garabaya , Santiago Cal , Carlos López-Otı́n

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  16. Nature. 2005 Sep 22;437(7058):564-8. Epub 2005 Aug 3.
    Accelerated ageing in mice deficient in Zmpste24 protease is linked to p53 signalling activation.
    Varela I1, Cadiñanos J, Pendás AM, Gutiérrez-Fernández A, Folgueras AR, Sánchez LM, Zhou Z, Rodríguez FJ, Stewart CL, Vega JA, Tryggvason K, Freije JM, López-Otín C.
    Author information
    Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncología, Universidad de Oviedo, 33006 Oviedo, Spain.

    Much more similar than you would expect.
    See: https://i.imgur.com/Ttj0XoS.png

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  17. According to “Fundación GADEA por la Ciencia”, http://gadeaciencia.org/en/the-foundation/board/
    of which the President Scientific Advisory Counsil (website’s typo), is the prominent scientist Mariano Barbacid, and the scientific advisory council http://gadeaciencia.org/en/scientific-advisory-council/includes of which includes such prominent members as:-
    Out of Spain section.
    1. Carlos Cordon-Cardo, (2017 retraction of 2003 paper http://mcb.asm.org/content/37/18/e00365-17.short “Digital experts have informed us that some of the published data in this paper were very likely digitally manipulated.”, but could not figure it out himself, which does suggest the question of his being about to advise anybody else about authenticity, or being able to judge data),
    2. Salvador “4 retractions” Moncada, who had 3 retractions in his group at UCL, https://retractionwatch.com/2012/09/04/three-retractions-for-university-college-london-mitochondrial-biologist-for-inappropriate-figure-manipulations/, under somebody’s nose does not suggest competency at being a judge, and one retraction delayed 17 years at Harvard. https://retractionwatch.com/2012/09/10/ori-findings-lead-to-two-retractions-nearly-17-years-later/, this particular retraction: http://www.jimmunol.org/content/189/6/3260.full
    In Spain section.
    3. Manel Esteller, who is well documented at Pubpeer:
    https://pubpeer.com/search?q=manel+estellerpubpeer
    3. Pura Muñoz-Canoves, who is also well-documented at Pubpeer:
    https://pubpeer.com/search?q=Mu%C3%B1oz-Canoves

    Last, but not least. Carlos Lopez-Otin.

    Carlos Lopez-Otin
    http://gadeaciencia.org/teams/lopez-otin-carlos-2/
    “The work of López-Otín’s laboratory has yielded the identification of more than 60 novel human proteases dysregulated in cancer and defined their functional role in cancer and in other pathological processes, including the devastating syndromes of accelerated aging.”
    His data are in conflict with those claims.
    It is not obvious if being a “Team” means that you are funded by “Fundación GADEA por la Ciencia”.

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  18. Cancer Res. 1996 Apr 15;56(8):1746-50.
    Cloning and expression analysis of human bleomycin hydrolase, a cysteine proteinase involved in chemotherapy resistance.
    Ferrando AA1, Velasco G, Campo E, Lopez-Otin C.
    Author information
    Departmento de Bioquimica y Biologia Molecular, Facultad de Medicina, Universidad de Oviedo, Spain.

    See: https://i.imgur.com/kBKnOAQ.png

    Like

  19. Blood. 1997 Jan 1;89(1):272-80.
    Deletions and loss of expression of p16INK4a and p21Waf1 genes are associated with aggressive variants of mantle cell lymphomas.
    Pinyol M1, Hernandez L, Cazorla M, Balbín M, Jares P, Fernandez PL, Montserrat E, Cardesa A, Lopez-Otín C, Campo E.
    Author information
    Department of Anatomic Pathology, Hospital Clinic Provincial, University of Barcelona, Spain.

    Figure 1. Differential splicing between panels dilutes meaning.
    See: https://i.imgur.com/znY6avC.png

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