Lawyering-up Medicine Research integrity

Aguzzi and the Lowlifes

The prion researcher Adriano Aguzzi used to describe his Pubpeer critics as "lowlifes", and himself as a victim of a lynch mob. But after Elisabeth Bik helped him find even more mistakes in his papers, Aguzzi changed his stance.

Adriano Aguzzi is prion researcher, director of the Institute for Neuropathology at the University Hospital (Universitätsspital) Zürich in Switzerland, member of several academies and a self-professed champion of publication ethics. Critics of his papers are described as a lynch mob and “lowlifes”, because apparently all those data irregularities do not matter in the greater scheme of things. Aguzzi’s prion research namely saved many lives. Or so he says.

The image integrity sleuth Elisabeth Bik now followed up on some year-old whistleblower concerns once posted on PubPeer. Back then, Aguzzi and his coauthors reacted with aggressive insults towards his critics. That said, every group leader can unknowingly become victim of a rogue PhD student or postdoc. But evading responsibility when faced with evidence of data manipulation, refusing to correct the scientific record while viciously attacking your critics is not an ethical response. Some define it as research misconduct. It seems, however, that Aguzzi has changed his stance now.

Update 14. and 21.12.2019: Aguzzi now announced to sue me for defamation. His inspiration comes from my sentencing in Berlin court over the deadly trachea transplants performed by Paolo Macchiarini and assisted by Philipp Jungebluth (the plaintiff). Aguzzi wrote this in the comment below:

I will have no option but a lawsuit. Get prepared for another conviction, and start saving for your legal expenses. You may learn your lesson this time.

Now Aguzzi made his threat official. He demands an immediate removal of this article and all tweets referencing him, my lifelong commitment never to say anything critical about him, otherwise he will pursue a lawsuit, with damage compensation of €15,000. A translation of this letter is provided at the end.

Readers of my site might recall that Aguzzi as panel member recently awarded the France-based German cancer and ageing researcher Guido Kroemer with the €1mn “Nobel Lombardo” (read here), despite or maybe actually because of Kroemer’s notoriety as peddler of Photoshopped research data. When awarding Kroemer, Aguzzi had no problem joining a quasi-all-male panel with the most toxic peer of all: Carlo Croce, with 9 retractions so far and a lost lawsuit against his critic David Sanders and the New York Times. To be fair, Aguzzi left the panel afterwards, officially to make room for women next year.

Aguzzi is advisory board member at Mercaptor. He has a PhD there, even in press releases.

Aguzzi’s world is weird. The Zürich professor occasionally claims having both MD and PhD degrees, but his own CV proves that he never did any PhD training, but graduated as MD in 1983, while on leave in USA during his medicine studies in Switzerland and Germany. In fact, Aguzzi explained that he sees his Doctor honoris causa (Dr h.c.) from University of Bologna as a kind of quasi-valid PhD degree. It is not really the same, years of researching in a lab and defending a doctorate thesis versus dressing up in a fancy hat for a party organised by admiring peers.

Update 27.12.2019. Aguzzi insists his Bologna degree is a PhD, even if a honorary one. Actually, what his own diploma says is that he received a “diploma di Laurea”, which is equivalent either to a Bachelor or a Master degree. In Italy, laurea graduates are traditionally addressed as “dottore” (or dottoressa), but a PhD degree is called in Italy “dottere di ricerca”, and is not to be confused with the dottore of the laurea. Hence, according to the diploma he posted, Aguzzi seems to have only a BSc h.c. or a MSc h.c. title from the University of Bologna, and not the honorary PhD at all.

Aguzzi’s laurea h.c. diploma, as posted by Aguzzi himself on Twitter

But now, let’s discuss Aguzzi’s scientific record. It seems, his worst enemies are the envious lynch mob “lowlifes” who discuss his papers on PubPeer. Like here:

Johannes Haybaeck , Mathias Heikenwalder , Britta Klevenz , Petra Schwarz , Ilan Margalith , Claire Bridel , Kirsten Mertz , Elizabeta Zirdum , Benjamin Petsch , Thomas J. Fuchs , Lothar Stitz , Adriano Aguzzi Aerosols transmit prions to immunocompetent and immunodeficient mice PLoS Pathogens (2011) doi: 10.1371/journal.ppat.1001257

In July 2013, an anonymous PubPeer critic voiced these concerns:

Figure 6D: Splice between lanes 6 and 7.

Figs 3 and 4 use four differently cropped, exposed and rotated images for the JH-/- (Fig. 3) and c3c4-/- (Fig. 4) mice. Is one of these mice both the same?

Fig. 5E left lane has been spliced onto the gel.

Fig. 5G right panel. Oddly blotchy pale, pixellated background with less detail than for the dark matter in the lanes. Multiple straight line effects at edges of bands. This gel appears to have been composed from multiple individual components inserted into a lower resolution background.

Fig. 6C left panel. Right vertical edge has a jaggie revealing that a shorter lane has been spliced in.

Fig. 6D right panel. strong splice between lanes 6/7″

The commenter also wondered if it made sense to declare that every single author has contributed everything to the paper.

Aguzzi immediately announced to investigate, and promised to share the results:

My policy is that all raw data be kept securely for at least 10 years, hence I foresee no problems with data retrieval. I will post the results of the audit.

More than two years, passed, and in November 2015 Aguzzi replied again:

I desisted from my original intent to publish further materials on this forum once it became clear that some of the self-appointed “peers” are more interested in mud-slinging than in post-publication review and quality control of science. I see no need to defend my work against a group of anonymous detractors who negate a priori my integrity, yet are not even prepared to identify themselves. Suffice it to say that Mathias Heikenwälder and I have gone carefully through all original data and we confirm that none of the alleged “manipulations” were performed. We stand fully by the findings which we have reported. Both Mathias and I will be prepared to make additional materials available to whoever will contact us directly and identify himself/herself, be it the Editor of PLoS Pathogens or anybody else. What we are NOT going to do is to engage in shouting match with anonymous individuals who make libelous and unfounded accusations. Please note that I will NOT make any further comments or communications on this forum.

The anonymous commenters pleaded with Aguzzi to reply again, and eventually the paper’s first author Johannes Haybäck (now director of Institute of Pathology at the University of Magdeburg in Germany, with a second affiliation at University of Graz in Austria), did:

I apologize for not having reacted earlier, but I had not understood the precise nature of the issue with the contested histology figures. I agree with that a mistake may have occurred while preparing the histology panel for publication. These are BSL4 experiments, which are carefully documented for reasons of both biosafety and animal welfare. I have planned to travel to Zurich on Dec. 7th in order to re-inspect all histology together with a coworker from the Aguzzi lab. The paraffin blocks from which the histology was prepared are certainly still existent, and I am confident that the issue can be clarified. If indeed there are errors, a correction will be published in the PLoS Pathogens paper.

In February 2015, PLOS Pathogens issued a Correction, for figures 3, 5 and 6. On PubPeer, Aguzzi then posted a lengthy comment, where he shared his views on anonymous post-publication peer review. He regretted that his “former student Johannes Haybäck committed an error while mounting Fig. 3A” , but saw little responsibility on his own part. Instead, this:

Now, I have been active in research since 1982. Over the past 34 years, I have published >450 paper. Notwithstanding the error in Fig. 3A, the Haybäck paper is one of those that I am proudest of. Our discovery of aerosols as prion vectors has led to a significant reassessment of the safety procedures for lab work with prions. By drastically improving the safety of lab workers, this work will likely save human lives. […]

In summary, if my scientific legacy will turn out to be a better protection from occupational hazards for students, postdocs, and lab technicians, well, that is a legacy that was worth working for, and one that I am proud of.”

Well, it is not like lab personnel used to get sick and die from prion exposure in epidemic proportions until Aguzzi’s life-saving paper came along. Come to think of it, there never were any cases of a dangerous prion infection in the lab. Aguzzi was exaggerating his scientific legacy, again.

That same whistleblower flagged in July 2013 other Aguzzi papers on PubPeer, but not even corrections have happened. Like here:

Brady Michel , Adam Ferguson , Theodore Johnson , Heather Bender , Crystal Meyerett-Reid , Bruce Pulford , Adriana Von Teichman , Davis Seelig , John H. Weis , Glenn C. Telling , Adriano Aguzzi, Mark D. Zabel Genetic depletion of complement receptors CD21/35 prevents terminal prion disease in a mouse model of chronic wasting disease Journal of immunology (2012) doi: 10.4049/jimmunol.1201579 

Bik provided good visualisation of the problem, namely some very insolent reuse of western blot bands in Figure 4:

The last author Mark Zabel, prion researcher at Colorado State University in USA, replied immediately with this comment:

We have commissioned an independent forensic analyses of the figures in this manuscript and will update this post with a report when it is complete. For now, we are sharing all of the raw data images used for these experiments and to create the figure here https://www.sugarsync.com/pf/D6450680_0347642_9170813

We are committed to the highest scientific rigor and integrity in our research. We stand by the data and the conclusions drawn from them in the paper, and will submit an errata to this paper to update the figure if these duplications are confirmed.”

Another PubPeer critic however retorted:

the problem already exists in the raw data since the two first Ig H blots (page 7 of above pdf) are actually manipulated versions of the same blot.”

Zabel then provided his final decision in October 2014 that nothing at all wrong was with the paper. He explained that loading controls were irrelevant anyway, and even accused the whistleblowers of having fabricated evidence in Photoshop:

An independent analysis of the files and blots, as well as interviews with authors of the original paper, concluded that “duplication or manipulation of raw blots was deemed unlikely, unintentional or inconclusive. Explanations given by the technicians who performed the experiments are plausible and no further action was recommended. In fact, it was demonstrated that unrelated blots could actually be made to look extremely similar by the same manipulations that raised concern about the images published in the manuscript.

In short, it could not be determined definitively whether these images were inadvertently duplicated blots, but that it was unlikely that any intentional manipulation of the blots had occurred. Moreover, the bands in question were mainly controls, which would not have significantly altered data interpretation. We therefore stand by the data and the conclusions drawn from them in the manuscript.

Of course the paper was not corrected, and rest assured it never will be. Journal of Immunology made it their business model of doing nothing at all about falsified research (read here and here). How else can infectious diseases be cured if not with the modern technology of Photoshop?

Another coauthor of Aguzzi was more constructive, after Bik flagged a badly manipulated figure. Background patches were copy-pasted, presumably to cover up results which stood in the way of the fight against a deadly infectious prion disease.

Ciriaco Ligios, Maria Giovanna Cancedda , Antonello Carta , Cinzia Santucciu , Caterina Maestrale , Francesca Demontis , Mariangela Saba , Cristiana Patta , James C DeMartini , Adriano Aguzzi, Christina J Sigurdson Sheep with scrapie and mastitis transmit infectious prions through the milk Journal of Virology (2011) doi: 10.1128/jvi.02022-10

Christina Sigurdson of UC San Diego in USA replied 2 days later:

We are looking into these blots as a high priority.”

Let’s hope Sigurdson will not pull a Zabel on this one, and that Journal of Virology is more ethical than Journal of Immunology, which is actually not that difficult. In the next case however, it is perfectly clear that the society journal Blood is not interested in a correction: Blood has a policy of ignoring everything older than a year or so, regardless of how bad. Hence, no need for Aguzzi or his Universitätsspital Zürich colleagues to bother about this reused and shifted image:

Stefan Baenziger , Mathias Heikenwalder , Pål Johansen , Erika Schlaepfer , Ursula Hofer , Regina C. Miller , Simone Diemand , Kenya Honda , Thomas M. Kundig , Adriano Aguzzi , Roberto F. Speck Triggering TLR7 in mice induces immune activation and lymphoid system disruption, resembling HIV-mediated pathology Blood (2009) doi: 10.1182/blood-2008-04-151712

“The polyl:C/F4.80 panel in Figure S2B (21 days) appears to show an overlap with the polyl:C/F4.80 panel in Figure S3B (42 days)”

Being such an important man, and likely unbothered by the journals, Aguzzi so far saw no reason whatsoever to address the concerns posted in parallel in 2013 regarding this paper:

Megan Larson , Mathew A Sherman , Fatou Amar , Mario Nuvolone , Julie A Schneider , David A Bennett, Adriano Aguzzi, Sylvain E Lesné The complex PrP(c)-Fyn couples human oligomeric Aβ with pathological tau changes in Alzheimer’s disease Journal of Neuroscience (2012) doi: 10.1523/jneurosci.1858-12.2012 

“The two top right panels in Figure 2B share many similarities. Thin red circles and ellipses highlight specks and spacings patterns between lanes with characteristic shapes and positions. “

Also the last author Sylvain Lesné of University of Minnesota in USA did not reply. Maybe he or Aguzzi will now, after Bik provided some illustrations? Or is this evidence also falsified in Photoshop, like previously uncovered by Zabel? How is this approach to curing Alzheimer’s to explain:

“two areas, highlighted with yellow boxes, look much, much more similar than expected.
Thin magenta arrow. A sudden, sharp vertical line between the two right-most lanes suggests an undisclosed splicing.” “similarity between figure 2D, lane 1 and Figure 4B left panel, as shown with thick blue boxes.”

Bik’s concerns about this Nature paper from Aguzzi lab were so far not dignified with a comment:

Michael B. Fischer , Christiane Roeckl , Petra Parizek , Hans Peter Schwarz , Adriano Aguzzi Binding of disease-associated prion protein to plasminogen Nature (2000) doi: 10.1038/35044100

A paper containing such a falsified figure would not be not reliable anymore. Falsifying data in Photoshop, regardless which of the authors did it, does affect the conclusions, and it is the responsibility of the corresponding author to act upon the evidence. It does not matter how many lives Aguzzi thinks he has saved with his prion research.

Update 13.12.2019. Aguzzi has discussed this Nature figure in the comment section below, and now first author Michael Fischer replied by email:

We had been able to answer questions about the paper to the satisfaction of everyone (Nature and University of Zurich) 4 years ago. I therefore do not want to comment again.

Aguzzi did reply to this commenter, who raised unspecified concerns about “Figures 1, 3, 4, 5” in March 2017. The first author was meanwhile finally tasked with retrieving raw data.

Caihong Zhu, Uli S. Herrmann , Jeppe Falsig , Irina Abakumova , Mario Nuvolone , Petra Schwarz , Katrin Frauenknecht , Elisabeth J. Rushing , Adriano Aguzzi A neuroprotective role for microglia in prion diseases The Journal of Experimental Medicine (2016) doi: 10.1084/jem.20151000

Back in 2017, the PubPeer critic also voiced concerns about shifted baselines, high mortality among the alleged healthy controls mice, and also this bizarre approach to statistics:

Experiments are largely underpowered: serve as examples N=4 or 3 from figures 3, 4, which are central to the argument of the paper. The dispersion of the data indicates extensive variability, calling for a substantial increase of the N. I am very surprised these datasets comply with the requirements for normality and homocesdasticty needed for the ANOVA“.

Original band photo, borrowed and modified for satire purposes, here.

The editors and reviewers at the elite journal somehow missed all that, or just trusted Aguzzi to know what he is doing. Aguzzi’s reply arrived years later, in July 2019, typos his:

The sentence “I wonder if somebody more expert could have a look at westerns at Figures 1, 3, 4, 5? Some of them look odd to me, but I’m not an expert in picking up image manipulation” provides a vivid demonstratin of everything that’s wrong with PubPeer.

The commenter is unhappy with this publication, most likely because he has some personal beef with one of the authors. However, as much as he would wish to prove the paper wrong, he cannot find any flaws in the figures. Therefore, he claims that they “look odd” to him, hoping that the mob will rise and lynch the authors – or at the very least that something disreputable will stick.

Sadly (but unsurprisingly), the world of research is not devoid of lowlifes. The meanness, spite, the posturing and the defamation under the protection of anonymity, you find them on Reddit and you find them on PubPeer as well.

That professorial reply to the lynch mob “lowlifes” actually provides a vivid demonstration of everything that’s wrong with Aguzzi. The statistics concerns are actually very much valid, and as for western blots: the loading controls do not always look like they show same gel. Despite Aguzzi et al having written in the methods section:

“To avoid variation in loading, the same blots were stripped and incubated with an anti-actin antibody”

That was apparently a fib, or an honest error of oversight, for the noble purpose of saving lives. Like here, in Figure 5, where an grid was placed to visualise that, sadly, and unlike Aguzzi et al explicitly wrote, GFAP signal and Actin loading control did not derive from same gel:

” With the risk of being labled as a lowlife or part of a lynch mob I would suggest that perhaps the oddity referred to by the original commenter is that some of the gels seem to radically change shape between stainings.”

But as we learned before from Aguzzi’s colleague Zabel, loading controls are irrelevant anyway, presumably in prion research at least. Although, Figure 1E does contain undisclosed splicing, in a paper from 2016:

Aguzzi eventually understood that maybe it was he who was wrong. The gels are possible not what they are presented to be. Hence this new reply:

I agree that this is weird. I’ll try to retrieve whatever original materials are around. My former postdoc Caihong Zhu has recently moved to Shanghai, but his records are meticulous and we are in touch every week. In fact we are still doing a lot of experiments together and he is still maintaining a large mouse colony in my institute. I am confident that he can shed light on this issue.”

With a paper barely 3 years old, the raw data should be available. Different with Aguzzi’s 11 year old paper, flagged by Bik for some very naughty Photoshoppery aimed at extirpating prion disease transmission.

Nicolas Genoud , David Ott , Nathalie Braun , Marco Prinz , Petra Schwarz , Ueli Suter , Didier Trono , Adriano Aguzzi Antiprion prophylaxis by gene transfer of a soluble prion antagonist American Journal Of Pathology (2008) doi: 10.2353/ajpath.2008.070836

Such things as in Figure 1C and 5h do not happen by mistake, those are intentional data manipulations. Regardless of who did it, as as corresponding last author and lab head Aguzzi is very much responsible for what he publishes. And no, having published many papers in Science, Nature and Cell is not an excuse.

Aguzzi warned however:

Thank you for pointing out these issues. I agree with your concern and I will try to retrieve the original histology slides. I cannot promise that I will find anything though. These experiments were performed >15 years ago. My policy is to retain all original materials for at least 10 years. Older materials have been occasionally retained, but not in a systematic manner.

Aguzzi also replied in similar vein on another paper, where an image was duplicated without change. That might be one of the rare cases of an honest mistake.

Update: Aguzzi now provided hi-res Figure 1 of the Genoud et al 2008 paper in a comment below. It makes the matter worse, there are even more manipulations in that figure than previously assumed.

Mathias Heikenwalder , Christian Federau , Lotta Von Boehmer , Petra Schwarz , Mareike Wagner , Nicolas Zeller , Johannes Haybaeck , Marco Prinz , Burkhard Becher , Adriano Aguzzi Germinal center B cells are dispensable in prion transport and neuroinvasion Journal of Neuroimmunology (2007) doi: 10.1016/j.jneuroim.2007.09.022

Aguzzi now announced to recover original data and correct the literature. Great start! He also promised to stop calling himself a PhD (something he used to do even in papers and book chapters as corresponding author).

Aguzzi also claims a PhD in Austria, where he is foreign Academy member.

Update 12.12.2019. For completeness, I decided to discuss this collaborative paper from Christian Haass lab at LMU Munich in Germany, coauthored by Aguzzi.

Armgard Uelhoff , Jörg Tatzelt , Adriano Aguzzi , Konstanze F. Winklhofer, Christian Haass A pathogenic PrP mutation and doppel interfere with polarized sorting of the prion protein Journal of Biological Chemistry (2005) doi: 10.1074/jbc.c400560200 

What we see here, is a western blot experiment where the “no signal” analysis was probed on one gel (Dpl in A and PrP in B), but the “with signal” analysis was made on a different, or maybe even several different gels, spliced together. There is therefore no positive control or evidence that any protein at all was loaded on the “no signal” gel. The authors might want to correct this figure, which is otherwise meaningless.

Before reading Aguzzi’s comments below, you might want to prepare yourself with this eulogy in Tagesanzeiger.

“A postdoc of his held last week a lecture at the Institute of a Nobel laureate in Israel, as the neuropathologist Adriano Aguzzi posted recently on Facebook. After the talk, the Nobel laureate told the postdoc: “Adriano should have received the Nobel Prize together with Stanley Prusiner”. This is not correct, Aguzzi continues in his post, “but my ego grew enormously, today it does not fit through the door anymore. “

The article continues to decry the tragedy that Aguzzi was not given the deserved Nobel Prize.

Update 16.12.2019. Another Aguzzi paper is discussed on PubPeer:

M Nuvolone , N Schmid , G Miele , S Sorce , R Moos , C Schori , RR. Beerli , M Bauer , P Saudan , K Dietmeier , I Lachmann , M Linnebank , R Martin , U Kallweit , Ve Kana , EJ. Rushing , H Budka , A Aguzzi Cystatin F is a biomarker of prion pathogenesis in mice PLoS ONE (2017) doi: 10.1371/journal.pone.0171923

9 years lie between these papers, is the loading control for Cst7 still valid? Aguzzi however says that Cst7 blot was done already back then, but unpublished. Saved for the distant future.

Aguzzi’s reply was:

It is simply one of the many attacks ad personam that Leonid Schneider (who was recently convicted and sentenced to a stiff pecuniary fine for slander by a German court, and was in addition ordered to pay all legal expenses to the person whom he slandered) is launching against me.

I could ask PubPeer to remove the slanderous comments by Schneider and others. But I will not, because I prefer everybody to see and judge for themselves the level of absurdity, incompetence and meanness of his latest smear campaign. Schneider, don’t delude yourself that I will not fight back.”

Aguzzi sees every attempt to critically discuss his papers as a personal attack, a smear campaign and slander, for which he threatens to sue his critics. This is unfortunately a kind of retaliation which every whistleblower has to deal with. Not sure what kind of signal University of Zürich wants to be sending here.

Update 21.12.2019.

Aguzzi sent me today me this registered letter, the original is shown at the beginning of the article:


Dear Mr. Schneider

You spread untrue claims about me and my employees. I request of you now, informally and extrajudicially, the following acts:

  • Please delete your blog entry “https://forbetterscience.com/2019/12/12/aguzzi-and-the-lowlifes”
  • Please delete all of your tweets and other online posts which mention my person and my employees
  • To refrain lifelong from mentioning my person in the future, from judging, attacking, hinting, speaking of, coercing, and publicising in any form online or otherwise.

This letter is meant as a friendly, unprejudiced request. If you comply with this request I will consider this unfortunate matter as settled and will undertake no further steps.

However, should you do not comply with my request within the next 10 days, you will receive from my legal representative a cease and desist declaration with penalty clause.

As you know from your previous experiences, this will be combined with compensation demands which correspond to the severity of your law violation. We envision a compensation of €15,000, which we intend to donate to a charitable foundation. You probably also know that repeat offenders can expect little understanding from the judiciary.

I sincerely hope that you recognize my benevolent willingness to settle out of court, will comply with my request and save you a lot of trouble.

Sincerely

Adriano Aguzzi

Update 24.12.2019

Christian Schwarzenegger, Univerity of Zürich’s Vice-Rector for faculty affairs and law professor, informed me on 22 December 2019 that the university is not involved with Aguzzi’s announced legal action against me. He however declared:

the university leadership has initiated an investigation of the accusations you raised. The procedure is as usual based on the presumption of innocence. As soon as we have a report, we will inform you about the next steps.”

Update 22.05.2020

Aguzzi has now been threatening a peer reviewer of his manuscript currently submitted to a journal. The original tweet, where Aguzzi mentioned some gel splicing, has been deleted. But here are screenshots and his follow-up comments:

The questionable Twitter behaviour prompted some PubPeer users to have another look at Aguzzi papers:

AM. Thackray , MA. Klein , A Aguzzi , R Bujdoso Chronic Subclinical Prion Disease Induced by Low-Dose Inoculum Journal of Virology (2002) doi: 10.1128/jvi.76.5.2510-2517.2002

Whoever did that gel, has a lot to answer for. But also the senior authors liek Aguzzi and Raymond Bujdoso in Cambridge should they think it is not a problem. Another problematic paper was done in collaboration with Max-plank-Institute in Frankfurt, Germany: Heber et al J Neuroscience 2000, other works are straight from Aguzzi’s lab though: Polymenidou et al PNAS 2004 and the vintage paper with his mentor and patron Charles Weissmann, Brandner et al PNAS 1996.


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137 comments on “Aguzzi and the Lowlifes

  1. Rex Rictor

    I agree complete with both “me again” and owlbert. Will get back tomorrow with longer message!

    Best rr

    Like

  2. Adriano Aguzzi

    Werter Herr Schneider,

    Sie sollten sich dessen bewusst sein, dass Sie als Verantwortlicher Im Sinne Des Presserechts für den von Ihnen herausgegebenen und moderierten Blog gerade stehen. Das heisst, Sie werden nicht nur für Ihre eigenen Behauptungen sondern auch für die Verbreitung von rufschädingenden Unwahrheiten durch Dritte zur Verantwortung gezogen, sofern diese Unwahrheiten auf Ihrem Blog erscheinen.

    Es ist leider so, dass die ursprünglichen Versionen Ihres Blogs alle noch verfügbar sind (webcache). Ausserdem habe ich eine automatische email von jedem Ihrer Kommentare erhalten und gespeichert. Es nützt also nichts, dass Sie nun Ihr Blog revisioniert haben.

    Mein Rechtsvertreter ist Dr. Gerrit Hötzel, der Ihnen bestens bekannt ist. Sie werden in den nächsten Tagen Post von Dr. Hötzel bekommen.

    Hochachtungsvoll
    Adriano Aguzzi

    Like

    • Mr Aguzzi, why do you claim your Bologna diploma certifies a Dr hc (or honorary PhD degree)?
      It says it grants you an honorary “diploma di Laurea”. Which is equivalent to a BSc or MSc degree (honorary in this case).
      A PhD in Italy is dottore di ricerca, and is never to be confused with dottore, which is what everyone with a laurea degree is traditionally called. It seems not even in Italy do you have an honorary PhD.

      Like

  3. I found wikipedia useful on how carefully use the title ‘honorary degree’:
    https://en.wikipedia.org/wiki/Honorary_degree

    Like

  4. You can apply for a honorary Doctor of Science online here. They will mail it to you ASAP (for a fee).
    https://sites.google.com/site/honorarydegreeacademics/honorary-doctor-of-science-d-sc-how-to-apply

    Liked by 1 person

  5. Mr Aguzzi,

    I think it would be valuable to update the page of declaration of potential conflict of interest, under letter A of corresponding University of Zürich website https://www.uzh.ch/prof/apps/interessenbindungen/client/A, such as:

    October 2018: Mr. Aguzzi is appointed at Mercator Discoveries as scientific advisor
    https://www.prnewswire.com/news-releases/mercaptor-announces-the-appointment-of-world-renowned-neuropathologist-dr-adriano-aguzzi-to-scientific-advisory-board-300737757.html.

    The sentence:”Mercaptor’s approach to treating neurodegeneration by addressing reactive oxygen species (ROS) parallels Dr. Aguzzi’s research’ is somehow worrying without any transparent public explanation of possible conflict of interest.

    September 2019: Mr Aguzzi is appointed as scientific advisor at ClearLight Biotechnologies, that bought the license of Crystal invention ”
    https://www.prnewswire.com/news-releases/clearlight-biotechnologies-signs-an-exclusive-license-agreement-to-develop-and-commercialize-the-crystal-technology-invented-at-the-university-of-zurich-and-the-university-hospital-zurich-300914758.html

    The sentence ” ClearLight Biotechnologies, LLC announced today the execution of an exclusive world-wide license agreement to develop and commercialize the 3D tissue clearing technology, CRYSTAL, invented by Professor Adriano Aguzzi, M.D, Ph.D” is somehow worrying without any transparent public explanation of possible conflict of interest.

    Thank you in advance for the update of the relevant University of Zürich website, and/or blogpost for clarification.

    Like

  6. “CRYSTAL, invented by Professor Adriano Aguzzi, M.D, Ph.D” Those of us with real degrees should sue this clown for diluting the PhD brand.

    Like

  7. Could he really be an accordion player? Check this link out. Imagine if he takes his glassess and hat off.

    Like

    • Not sure I got what is this video about. There are two guys faking accordion play. (The music is playback.) How does it relate to the thread?….

      Like

      • David Dignam

        I guess the music is to similar to Adriano Aguzzi’s science.

        Dreadful.

        Like

  8. Bullying has become gender neutral. Please read the bullying of Stefan Grimm and what happened to him. Very sad.

    http://www.dcscience.net/2014/12/01/publish-and-perish-at-imperial-college-london-the-death-of-stefan-grimm/

    Like

  9. Cluster of problematic prion publications.

    https://pubpeer.com/search?q=ruth+gabizon

    Like

    • The different authors and the frequency of blot manipulations suggest that the common last author Prof. Gabizon does not take blot integrity seriously. Within such culture, group members easily shift from beautification to minor misconduct to major misconduct as seems to be shown in this set of pubpeer reports.

      As I mentioned before, in my opinion, in the works by Prof. Aguzzi there is no such “signature” of a flawed attitude of the group leader. But yes, he should have dealt better with integrity concerns.

      Like

  10. It was interesting to follow this exchange. In this light it is worth looking at what was said by Adriano Aguzzi about others research:

    http://precedings.nature.com/documents/3344/version/1

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  11. I just found this on Twitter. It’s interesting how Aguzzi is approaching to new complains of misconduct during peer-review. He’s demanding that the Editor contact the institution of the ANONYMOUS reviewer???

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  12. Pingback: Michael HotTiger of Zurich, patron of biomedical ethics – For Better Science

  13. Imagine this level of plagiarism: “abstract, discussion, figure legends, and figures”
    https://pubpeer.com/publications/104AFBB99819E23347C0BACE069DDA

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  14. Anonymous for not having money to be sued

    Dear Leonid, please publish this comment in the section that youn consider more appropriate.

    I spent some years in Aguzzi’s lab during part of his golden years, I published with him and coexisted with most of the people that you referred in your previous comments.

    I share with you some small stories

    Scientifically many colleagues strongly wondered about the accuracy of the results of the colleague Mathias Heikenwalder, which was the most prominent researcher of Aguzzi’s group at that time. This was due to his psychologic profile and to the many privileges he enjoyed, that protected from internal scrutiny of his data. Some of the privileges were the following:

    He was the biosafety responsible for the animal experiments, and therefore he could demand every colleague to tell him about his animal experiments, but on the contrary, no one had the power to control in detail Mathias experiments, except for Aguzzi, which of course had no time for such micro-management.
    One evidence of the dominant role of Matthias Heikenwalder in the animal experiments is his co-authorship in almost all the publications from Aguzzi’s lab that involved inoculation of mice.
    Since the researchers had to sacrifice the mice before they were actually dead (due to the rules of the swiss veterinary office that imposed the minimization of the unnecessary suffering from the animals and also in order to allow for the consequent forensic analysis), we generally questioned on that Matthias’s decision on the time for sacrificing the mice and consequently the registry of their survival time. One fact that that promoted these doubts was the fact that Mathias often did both the inoculation of the mice, their marking and their sacrifice and therefore his experiments were not blind.
    To this topic I recall a popular lab meeting in which one notorious colleague insisted with Mathias on “why don’t you use your mice of your popular publication (xyz) as a positive control in this experiment, since you already know and published the effect on them?”, to which it followed a discussion that few could follow, and that was closed by Aguzzi showing the support to Mathias, that such positive control was not relevant.
    Aguzzi had one of the biggest collections of transgenic mice and Mathias always took advantage of that collection and of the freedom for lavish expenses with his animal experiments to use very complex combinations of transgenic mice. Since the labs are often not willing to share very unique transgenic mice and since the combination of transgenic mouse lines used in the experiments was high, it was and it is still very difficult for other labs to reproduce the experiments.
    Furthermore internally we often commented that even if someone managed to get the mice and try to reproduce the experiments, Matthias could always allege that eventual non-reproducibility was due to the different inoculae. And here is the key point: the inoculae were a mixture of brain homogenates and showed differences from lot to lot. And a lot of inoculum was used up within about 3 years and I do not think that sufficient amounts of old inocullum were preserved for eventual experiment replication.

    From Mathias’s papers, I highlight the following that would deserve further scrutiny:

    This one from Marco Prinz, that was the scientific coach of Mathias Heikenwalder (second author) at Agguzi’s lab. This is a critical paper because it allowed for the allegedly full clarification of the infectious path of prions from the gut to the brain. However due to the complexity of transgenic mice, I personally wonder if was ever reproduced.
    https://pubmed.ncbi.nlm.nih.gov/14562059/

    This one from Mathias Heikenwalder that presents the typical case of a plausible story that may have been enhanced by showing perfect results. Again the complexity of transgenic mouse lines make this paper difficult to reproduce
    https://pubmed.ncbi.nlm.nih.gov/15661974/

    This one from Johannes Haybaeck and Mathias Heikenwalder, on a research project that internally lasted for many years – which suggest non-obvious results – and already demanded a correction of the paper. I personally wonder if the results are reproducible
    https://pubmed.ncbi.nlm.nih.gov/21249178/

    I highlight that internally, Aguzzi’s always clearly expressed his intolerance towards data manipulation. Furthermore I have the strong belief that Aguzzi would never allow the publication of erroneous results or with manipulated data – he would have much more to lose than to win.

    However I consider that Aguzzi chose not to scrutinize and even to protect from internal scrutiny some researchers that made him dream about high impact-factor publications.

    Consequently, without ever doing it directly, Aguzzi promoted the creation of good stories with little scrutiny.

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  15. Pingback: The Ballad of Claudio Hetz – For Better Science

  16. Like

    • My PhD is real though. Unlike the fake degree of a certain someone.

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